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Separation of proteins

  • US 5,728,559 A
  • Filed: 12/01/1995
  • Issued: 03/17/1998
  • Est. Priority Date: 07/09/1993
  • Status: Expired due to Term
First Claim
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1. A method for obtaining a crystallized microbial protein from an aqueous mixture of proteins, said method comprising of the steps of:

  • (a) obtaining a microbial culture broth having a mixture of proteins, comprising a desired protein selected from the group consisting of an oxidoreductase, a protease, a lipase, a cellulase and an amylase;

    (b) adjusting the salt concentration of said protein mixture at or below 1.5 Molar;

    (c) adding a water soluble polymer to the protein mixture of step (b);

    (d) stirring the polymer-containing protein mixture of step (c) for up to 48 hrs; and

    (e) recovering the desired protein in crystalline form from the stirred mixture of step (d).

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