Chemistry analyzer

• US 5,730,938 A
• Filed: 08/09/1995
• Issued: 03/24/1998
• Est. Priority Date: 08/09/1995
• Status: Expired due to Fees

• Current Assignee: Bio-Chem Laboratory Systems, Inc.
• Original Assignee: Bio-Chem Laboratory Systems, Inc.
• Inventors: Turpen, Jon D., Carbonari, Larry Alfred
• Primary Examiner(s): Le, Long V.
• Application Number:
  US08/512,894
• Time in Patent Office: 958 Days
• Field of Search: 422/63, 422/64, 422/67, 422/81, 422/100, 422/102, 436/43, 436/47, 436/48, 436/49, 436/50, 436/54, 436/174, 436/179, 436/180, 436/164, 73/864.22, 73/864.34, 134/170, 134/21, 134/171, 356/320
• US Class Current: 422/64
• CPC Class Codes: G01N 2035/0437 Cleaning cuvettes or reaction vessels
• CPC Class Codes: G01N 35/10 Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices G01N35/0099 takes precedence
• CPC Class Codes: G01N 35/1004 Cleaning sample transfer devices
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack
• CPC Class Codes: Y10T 436/114998 with treatment or replacement of aspirator element [e.g., cleaning, etc.]
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A carousel receives a plurality of removable reagent containers, a turntable receives a plurality of sample fluid containers and a rotatable cuvette assembly holds an annular array of reaction and test cuvettes. A robotic arm carrying a fluid transfer needle coupled to a pair of syringes picks up one or more reagents and sample fluid for deposit into a cuvette. As the arm moves the needle tip exterior is washed and contaminants sent to a waste collector. At the end of a test cycle, the needle core is flushed and cleaned. A colorimetry photometric test is performed on the reacted fluids in each cuvette by a system employing ten interference filters, corresponding diode detectors and amplifiers employing two identical multiplexers providing identical filtered signals for logarithmic calculation of absorbance. The calculation subtracts a noise base level signal of one filter output from one multiplexer from a peak level signal produced by a second filter output from the other multiplexer for each component under test. A reciprocating plunger aspirates and cleans each cuvette at a single cleaning station employing multiple reciprocating motions. The photometric test is completed on all cuvettes in a single test cycle which are subsequently cleaned for the next test sequence.

Claims

1. A chemical analyzer system comprising:

cuvette means including a plurality of cuvettes;

sample holding means for holding a plurality of samples;

reagent holding means for holding at least one reagent;

transfer means including a needle having an external surface and a hollow core insertable into and for receiving at least a portion of one of said samples and at least a portion of said at least one reagent and for transferring the received portions to one of said cuvettes, said transferring of each portion occurring in a period of a given duration, said received portions forming a test sample in said one cuvette;

needle washing means including means for washing the needle external surface continuously during substantially the entire duration of said transferring periods of said portions and for washing the needle core subsequent to said transferring periods of said portions;

cuvette cleaning means comprising plunger means including a single plunger for reciprocating within said one cuvette subsequent to said transferring for automatically washing, aspirating and drying the one cuvette during said reciprocating at one cleaning location;

colorimetric photometer means including light generating means and a plurality of filter means responsive to the light from the light generating means passed through the test sample for producing a plurality of signals each corresponding to a different filter means and manifesting a different wavelength of said passed through light; and

calculating means responsive to said signals for generating an output signal manifesting a calculated light absorbance value of said test sample.

2. The system of claim 1 wherein said transfer means includes means for transferring the needle to and from a home station, said needle washing means including means for washing the needle external surface while the needle is in motion between said home station and each said cuvette means, sample holding means and reagent holding means.

3. The system of claim 1 including a diluent fluid and vacuum means for creaking a vacuum, means for coupling the fluid and the vacuum to said plunger means and to said needle washing means for drawing said fluid into and out of said one cuvette and for drawing said fluid about said needle surface in response to said vacuum to effect said washing of said cuvette and needle surface.

4. The system of claim 3 including pump means for applying said fluid under pressure to said needle washing means.

5. The system of claim 3 including syringe means for applying fluid to said needle core for diluting said received reagent and sample during said transferring and for washing said core.

6. The system of claim 1 wherein said plunger means is for cleaning one cuvette at a time at one station location, each said cuvettes having a cylindrical cavity, the plunger comprising a cylindrical member having an outer peripheral surface and an end for insertion into said cavity of an associated cuvette, said inserted plunger and associated cuvette defining a region therebetween, said plunger having an axial bore in communication with said end of the plunger and a plurality of radial bores in communication with the axial bore and the outer peripheral surface of the plunger and means for applying a vacuum to said axial bore and for alternately applying wash fluid and air to the region between the plunger and the cuvette to effect alternating washing and drying of said one cuvette in respective aspirating, washing and drying reciprocating cycles at said one station location wherein each cycle corresponds to a selected one of said aspirating, washing and drying in response to said vacuum withdrawing said applied fluid and air in said region.

7. The system of claim 6 wherein the outer peripheral surface of the inserted plunger member defines a clearance space with the cuvette cavity for receiving said fluid and air, said means for applying said wash fluid and air including means for cyclically effecting said washing and drying of said one cuvette.

8. The system of claim 1 wherein said plunger means comprises a support, means for vertically reciprocating said support, means for securing the plunger to said support for reciprocating displacement therewith, said plunger having a hollow first core, a body having a hollow second core for movably receiving said plunger therein and resiliently secured to said support for vertical displacement with said support, a gasket secured to said body for sealing resilient engagement with said one cuvette in response to said reciprocating support and means for applying a negative pressure to said first core and for selectively fluid coupling one at a time air and a washing fluid to said second core.

9. The system of claim 8 wherein said second hollow core defines a fluid chamber surrounding said plunger, said chamber forming a continuous chamber with said one cuvette with said gasket engaged, and means for sealing said chamber between said body and said plunger distal said gasket so that the chamber remains fluid sealed while said plunger reciprocates within said chamber.

10. The system of claim 1 wherein said sample holding means comprises a plurality of tubes each for holding a different sample, said reagent holding means include a plurality of containers each for holding at least one reagent, each said cuvette means, sample holding means and reagent holding means comprising a rotatable carousel, and further including means for causing said transfer means to transfer a plurality of said samples and a plurality of said reagents to a plurality of said cuvettes in a sequence, said cuvette washing means includes means for washing, aspirating and drying each cuvette sequentially one at a time during said sequence.

11. The system of claim 1 wherein said filter means includes a plurality of filters each for generating a corresponding output signal manifesting a given component of said sample, first amplifier means for amplifying each said output signals, multiplexer means responsive to said output signals for generating at least one multiplexed signal and second amplifier means for amplifying said at least one multiplexed signal.

12. The system of claim 11 wherein said multiplexer means comprises first and second multiplexers each for receiving said output signals of each said plurality of filters, said at least one multiplexed signal comprising third and fourth simultaneously generated like multiplexer signals, said calculating means including means for subtracting the fourth signal from said third signal corresponding to each said filters to remove a noise component from said third signal and means for calculating said absorbance values.

13. A chemical analyzer system comprising:

cuvette means including a plurality of cuvettes;

sample holding means for holding a plurality of samples;

reagent holding means for holding at least one reagent;

transfer means including a needle having an external tip surface and a hollow core insertable into and for receiving at least a portion of one of said samples and at least a portion of said at least one reagent and for transferring the received portions to one of said cuvettes in a corresponding portion transfer cycle having a period of a given duration; and

needle washing means including means for washing the needle external surface during substantially the entire period of each said transfer cycles and for washing the needle core after the completion of all said cycles.

14. The system of claim 13 wherein said transfer means includes means for transferring the needle to and from a home station, said needle washing means including means for washing the needle external surface while the needle is in motion between said home station and each said cuvette means, sample holding means and reagent holding means.

15. The system of claim 13 including a diluent fluid and vacuum means for creating a vacuum, means for coupling the fluid and the vacuum to said needle washing means for drawing said fluid into and out of said hollow core and for drawing said fluid about said needle surface in response to said vacuum applied to said hollow core and needle surface to effect said washing of said core and needle surface.

16. The system of claim 13 wherein said needle washing means comprises a housing having a single central cavity and aligned first and second through bores in fluid communication with the cavity and the ambient atmosphere for receiving the needle, means for reciprocating said needle in the bores and said cavity and for selectively locating said needle in said cavity, and further comprising means for selectively applying a diluent fluid to said needle core and a vacuum and washing fluid to said cavity.

17. The system of claim 15 including syringe means for applying fluid to said needle core for diluting said received reagent and sample and for washing said core.

18. A chemical analyzer system comprising:

cuvette means including a plurality of cuvettes each having a cuvette cavity;

sample holding means for holding a plurality of samples;

reagent holding means for holding at least one reagent;

transfer means for transferring at least a portion of one of said samples and at least a portion of said at least one reagent to one of said cuvettes;

test means for testing the one sample combined with the at least one reagent for at least one parameter in said one cuvette; and

cuvette washing means including plunger means comprising sealing means for sealing said one cuvette cavity during washing of said one cuvette and a single plunger for reciprocating within said one cuvette cavity subsequent to said testing at one wash location of said one cuvette for automatically aspirating, washing and drying the one cuvette sealed cavity at the one cuvette location during said reciprocating in respective aspirating, washing and drying reciprocating cycles of the one cuvette at said one wash location wherein each cycle corresponds to a selected one of said aspirating, washing and drying.

19. The system of claim 18 wherein the plunger comprises a cylindrical member for insertion in said one cuvette cavity and defining a region in the cavity between the one cuvette and plunger, said plunger having an axial bore in communication with an end of the plunger and a plurality of radial bores in communication with the axial bore and the outer peripheral surface of the plunger and means for applying a vacuum to said axial bore and for alternately applying a wash fluid and air to the region between the plunger and the cuvette to effect said cyclically occurring washing, aspirating and drying of said one cuvette cavity.

20. The system of claim 19 wherein the outer peripheral surface of the inserted plunger member defines a clearance space in said region with the cuvette cavity for receiving said fluid and air.

21. The system of claim 18 wherein said sealing means includes gasket means for providing an airtight seal between said plunger means and said one cuvette.

22. In a chemical analyzer system comprising cuvette means including a cuvette, sample holding means for holding a sample and reagent holding means for holding at least one reagent, the combination comprising:

transfer means including a needle having an external tip surface and a hollow core for insertion into and for receiving at least a portion of one of said samples and at least a portion of said at least one reagent and for transferring the received portions to a cuvette of said cuvette means in a corresponding portion transfer cycle having a period of a given duration; and

needle washing means including means for washing the needle external surface continuously during substantially the entire period of each said transferring cycles and for washing the needle core after the completion of all said cycles.