High affinity TGF.beta. nucleic acid ligands
First Claim
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1. A method of identifying nucleic acid ligands to transforming growth factor beta (TGFβ
- ) comprising;
a) preparing a candidate mixture of nucleic acids;
b) contacting the candidate mixture of nucleic acids with TGFβ
, wherein nucleic acids having an increased affinity to TGFβ
relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to TGFβ
, whereby nucleic acid ligands of TGFβ
may be identified.
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Abstract
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to TGFβ. Included in the invention are specific RNA and ssDNA ligands to TGFβ1 identified by the SELEX method. Also included are RNA ligands that inhibit the interaction of TGFβ1 with its receptor.
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Citations
9 Claims
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1. A method of identifying nucleic acid ligands to transforming growth factor beta (TGFβ
- ) comprising;
a) preparing a candidate mixture of nucleic acids; b) contacting the candidate mixture of nucleic acids with TGFβ
, wherein nucleic acids having an increased affinity to TGFβ
relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;c) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and d) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to TGFβ
, whereby nucleic acid ligands of TGFβ
may be identified. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
- ) comprising;
Specification