Methods and systems for biological reagent placement

• US 5,731,152 A
• Filed: 05/13/1996
• Issued: 03/24/1998
• Est. Priority Date: 05/13/1996
• Status: Expired due to Fees

• Current Assignee: Amersham Biosciences (General Electric Company), William Reber LLC
• Original Assignee: Motorola, Inc. (Motorola Solutions, Inc.)
• Inventors: Maracas, George N., Ackley, Donald E., Reber, William L., Harvey, Thomas B. III
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): REES, DIANE
• Application Number:
  US08/648,635
• Time in Patent Office: 680 Days
• Field of Search: 435/6, 435/5, 435/7.1-7.9, 435/30, 435/287, 435/91.1, 435/91.2, 422/131, 536/22.1, 530/334, 530/333, 436/500
• US Class Current: 435/6.11
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00367 capillary
• CPC Class Codes: B01J 2219/00369 in multiple or parallel arrangements
• CPC Class Codes: B01J 2219/00382 Stamping
• CPC Class Codes: B01J 2219/00385 Printing
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 4/02 for feeding measured , i.e. prescribed quantities of reagents
• CPC Class Codes: B82Y 10/00 Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G03F 7/0002 Lithographic processes using patterning methods other than those involving the exposure to radiation, e.g. by stamping

Abstract

An apparatus for placing at least one biological reagent at a plurality of locations on a substrate includes a stamp member onto which the at least one biological reagent is applied. The stamp member defines a plurality of transfer elements patterned to correspond to the plurality of locations. The stamp member contacts the substrate to transfer the at least one biological reagent from the plurality of transfer elements to the plurality of locations. The transfer elements can be defined by reservoirs or projected portions of the stamp member. A method of using said apparatus is also disclosed.

Claims

1. A method of placing at least one biological reagent at a plurality of locations on a substrate, the method comprising the steps of:

providing a stamp member having a plurality of projected portions, each of the projected portions having at least one cavity therein;

applying the at least one biological reagent to the stamp member; and

contacting the stamp member with the substrate to transfer the at least one biological reagent from the stamp member to the plurality of locations.

2. The method of claim 1 wherein the stamp member is cylindrically-shaped.

3. The method of claim 2 wherein the step of contacting the stamp member with the substrate includes rolling the stamp member onto the substrate.

4. The method of claim 1 wherein the at least one biological reagent includes at least one nucleotide.

5. The method of claim 1 wherein the plurality of locations include a plurality of binding sites.

6. The method of claim 1 wherein the plurality of projected portions absorb the at least one biological reagent when the at least one biological reagent is applied to the stamp member.

7. The method of claim 6 wherein the plurality of projected portions are made from an absorbent material.

8. The method of claim 7 wherein the stamp member includes a support member which supports the absorbent material.

9. The method of claim 1 wherein the stamp member has a capillary disposed therein.

10. The method of claim 1 wherein the at least one biological reagent is applied to each of the plurality of projected portions.

11. The method of claim 10 wherein the common biological reagent is applied to the stamp member by a roller.

12. The method of claim 1 further comprising the step of selectively further projecting at least one of the plurality of projected portions.

13. The method of claim 12 wherein the at least one of the plurality of projected portions is electromechanically projected.

14. The method of claim 13 wherein each of the at least one of the plurality of projected portions is electromechanically extended by a respective piston in the stamp member.

15. The method of claim 14 wherein the stamp member includes an absorbent layer which is deformable by each respective piston.

16. A method of placing at least one biological reagent at a plurality of locations on a substrate, the method comprising the steps of:

providing a stamp member having a surface with a plurality of reservoirs disposed therein;

applying the at least one biological reagent to the stamp member; and

contacting the stamp member with the substrate to transfer the at least one biological reagent from the stamp member to the plurality of locations.

17. The method of claim 16 further comprising the step of maintaining contact between the stamp member and the substrate until a reaction which involves the at least one biological reagent has completed.

18. The method of claim 17 wherein the reaction includes a hybridization event.

19. The method of claim 16 wherein the stamp member includes a plurality of capillaries.

20. An apparatus for placing at least one biological reagent at a plurality of locations on a substrate, the apparatus comprising:

a stamp member onto which the at least one biological reagent is applied, the stamp member having a plurality of projections, each of the projected portions having at least one cavity, the stamp member for contacting with the substrate to transfer the at least one biological reagent to the plurality of locations.

21. The apparatus of claim 20 wherein the stamp member is cylindrically-shaped.

22. The apparatus of claim 21 wherein the stamp member is rolled onto the substrate to transfer the at least one biological reagent to the plurality of locations.

23. The apparatus of claim 20 wherein the stamp member has a surface with a plurality of reservoirs therein.

24. The apparatus of claim 23 wherein the at least one biological reagent includes at least one nucleotide.

25. The apparatus of claim 23 wherein the plurality of locations are binding sites in a molecular detection apparatus.

26. The apparatus of claim 23 wherein the plurality of projected portions absorb the at least one biological reagent when the at least one biological reagent is applied to the stamp member.

27. The apparatus of claim 26 wherein the stamp member includes an absorbent layer which includes the plurality of projected portions.

28. The apparatus of claim 27 wherein the stamp member includes a support member which supports the absorbent layer.

29. The apparatus of claim 23 further comprising a capillary embedded in the stamp member.

30. The apparatus of claim 23 wherein the at least one biological reagent is applied to each of the plurality of projected portions.

31. The apparatus of claim 23 further comprising means for selectively further projecting at least one of the plurality of projected portions.

32. The apparatus of claim 31 wherein the at least one of the plurality of projected portions is electromechanically projected.

33. The apparatus of claim 32 wherein the means for selectively further projecting the at least one of the plurality of projected portions includes a piston in the stamp member.

34. The apparatus of claim 33 wherein the stamp member includes an absorbent layer which is deformed by the piston.

35. All apparatus for placing at least one biological reagent at a plurality of locations on a substrate, the apparatus comprising:

a stamp member having a biological reagant application surface with a plurality of reservoirs disposed therein and a plurality of capillaries embedded into the stamp member.

36. The apparatus of claim 35 wherein contact is maintained between the stamp member and the substrate until a reaction which involves the at least one biological reagent has completed.

37. The apparatus of claim 36 wherein the reaction includes a hybridization event.

38. The apparatus of claim 35 wherein the plurality of locations are binding sites in a molecular detection apparatus.

39. A method of placing at least one biological reagent onto a substrate, the method comprising the steps of:

providing a stamp member having a plurality of projected portions, each of the projected portions having at least one cavity therein;

applying the at least one biological reagent to the stamp member using a roller, and

transferring the at least one biological reagent from the stamp member to the substrate.

40. A method of placing at least one biological reagent onto a substrate, the method comprising the steps of:

providing a stamp member having a surface with a plurality of reservoirs disposed therein;

applying the at least one biological reagent to the stamp member using a roller, and

transferring the at least one biological reagent from the stamp member to the substrate.

41. An apparatus for placing at least one biological reagent at a plurality of locations on a substrate, the apparatus comprising:

a stamp member having plurality of projected portions on a cylindrical surface each projected portion having at least one cavity therein, said plurality of projected portions capable of transferring the at least one biological reagent to a plurality of locations.

42. The apparatus of claim 41 wherein the cylindrical surface is rolled on the substrate to transfer the at least one biological reagent to the substrate.

43. A method of placing at least one biological reagent at a plurality of locations on a substrate, the method comprising the steps of:

providing a stamp member having a plurality of projected portions on a cylindrical surface;

applying the at least one biological reagent to the plurality of projected portions; and

transferring the at least one biological reagent from the plurality of projected portions to the substrate.

44. The method of claim 43 wherein the step of transferring includes rolling the cylindrical surface on the substrate.