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Bacillus-derived transglutaminase

  • US 5,731,183 A
  • Filed: 02/09/1996
  • Issued: 03/24/1998
  • Est. Priority Date: 02/09/1995
  • Status: Expired due to Term
First Claim
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1. A purified Bacillus subtilis-derived transglutaminase having the following physicochemical properties:

  • (a) active between about pH 7 and pH 9,(b) active between about 40°

    C. and about 65°

    C.,(c) stable at about 60°

    C. or lower,(d) enzymatic activity of the transglutaminase is independent of Ca2+ and has an activity of 50% or more in the presence of 5 mM of Ca2+,(e) it is inhibited by N-ethylmaleimide, cystamine or ammonium sulfate,(f) it is not inhibited by ethylenediamine-tetraacetic acid,(g) it has a molecular weight of (i) from about 18,000 to about 22,000 as measured by gel permeation chromatography and (ii) from about 28,000 to about 30,000 as measured by SDS-PAGE, and(h) it catalyzes the transacylation of the γ

    -carboxamide group in glutamine residue(s) in a peptide chain.

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