Enhancing bone marrow engraftment using MSCS

• US 5,733,542 A
• Filed: 01/24/1995
• Issued: 03/31/1998
• Est. Priority Date: 11/16/1990
• Status: Expired due to Term

• Current Assignee: Mesoblast International Srl
• Original Assignee: Case Western Reserve University
• Inventors: Gerson, Stanton L., Lazarus, Hillard M., Haynesworth, Stephen E., Caplan, Arnold I.
• Primary Examiner(s): Lankford, Jr., Leon B.
• Assistant Examiner(s): TATE, CHRISTOPHER ROBIN
• Application Number:
  US08/377,771
• Time in Patent Office: 1,162 Days
• Field of Search: 424/423, 424/422, 424/549, 424/577, 424/93.1, 424/93.7, 435/240.1, 435/240.21, 435/240.2, 435/325, 435/366, 435/372, 623/16, 623/11
• US Class Current: 424/93.7
• CPC Class Codes: A61F 2/28 Bones joints A61F2/30
• CPC Class Codes: A61F 2/30756 Cartilage endoprostheses A61F2/3603 takes precedence
• CPC Class Codes: A61K 2035/124 the cells being hematopoietic, bone marrow derived or blood cells
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61L 2430/02 for reconstruction of bones; weight-bearing implants
• CPC Class Codes: A61L 2430/10 for reconstruction of tendons or ligaments
• CPC Class Codes: A61L 27/3834 Cells able to produce different cell types, e.g. hematopoietic stem cells, mesenchymal stem cells, marrow stromal cells, embryonic stem cells
• CPC Class Codes: A61L 27/3843 Connective tissue
• CPC Class Codes: A61L 27/3895 using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
• CPC Class Codes: A61L 31/005 Ingredients of undetermined constitution or reaction products thereof
• CPC Class Codes: C07K 16/28 against receptors, cell surface antigens or cell surface determinants
• CPC Class Codes: C12N 2501/2301 Interleukin-1 (IL-1)
• CPC Class Codes: C12N 2501/39 Steroid hormones
• CPC Class Codes: C12N 2533/18 Calcium salts, e.g. apatite, Mineral components from bones, teeth, shells
• CPC Class Codes: C12N 5/0663 Bone marrow mesenchymal stem cells (BM-MSC)

Abstract

Method and preparations for enhancing bone marrow engraftment in an individual by administering (i) a culturally expanded mesenchymal stem cell preparation and (ii) a bone marrow graft. The mesenchymal stem cells are administered in an mount effective to promote engraftment of the bone marrow.

Claims

1. A method for enhancing bone marrow engraftment in an individual in need thereof which comprises administering to said individual (i) isolated, homogeneous mesenchymal stem cells and (ii) a bone marrow graft, wherein said mesenchymal stem cells are administered in an amount effective to promote engraftment of said bone marrow in said individual.

2. The method of claim 1 wherein the mesenchymal stem cells are administered by intravenous injection or by injection directly to the site of intended activity.

3. The method of claim 1 wherein at least one of (i) the mesenchymal stem cells and (ii) the bone marrow graft is administered intravenously.

4. The method of claim 1 wherein at least one of (i) the mesenchymal stem cells and (ii) the bone marrow graft is injected into said cavity.

5. The method of claim 1 wherein the mesenchymal stem cells are autologous.

6. The method of claim 1 wherein the mesenchymal stem cell preparation is administered concurrently with the bone marrow graft.

7. The method of claim 6 wherein the mesenchymal stem cells are introduced into said individual in a cell suspension also containing bone marrow graft cells.

8. The method of claim 1 wherein the mesenchymal stem cell preparation is administered to the individual prior to administration of the bone marrow graft.