Method and compositions for flow cytometric determination of DNA sequences

• US 5,736,330 A
• Filed: 10/11/1995
• Issued: 04/07/1998
• Est. Priority Date: 10/11/1995
• Status: Expired due to Term

• Current Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Original Assignee: Luminex Corporation (DiaSorin S.p.A.)
• Inventors: Fulton, R. Jerrold
• Primary Examiner(s): Zitomer, Stephanie W.
• Assistant Examiner(s): REES, DIANE
• Application Number:
  US08/542,401
• Time in Patent Office: 909 Days
• Field of Search: 435/6, 435/5, 435/210, 435/91.2, 435/7.1-7.9, 436/523, 536/24.3, 536/26.5
• US Class Current: 435/6.12
• CPC Class Codes: C12Q 1/6827 for detection of mutation or polymorphism
• CPC Class Codes: C12Q 2537/161 A competitive reaction step
• CPC Class Codes: C12Q 2563/149 Particles, e.g. beads
• CPC Class Codes: G01N 15/1012 Calibrating particle analysers; References therefor
• CPC Class Codes: G01N 15/1456 without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1014 Constitution of reference particles
• CPC Class Codes: G01N 2015/1477 Multiparameters
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 2015/1488 Methods for deciding
• CPC Class Codes: G01N 33/5094 for blood cell populations red blood cells G01N33/80
• CPC Class Codes: G01N 33/54313 the carrier being characterised by its particulate form

Abstract

A method for the analysis of DNA sequences and PCR products comprises the steps of constructing an oligonucleotide-labeled bead set, and labeled complementary probe, and exposing the bead set and probe to a DNA fragment or PCR product under hybridizing conditions and analyzing the combined sample/bead set by flow cytometry. Flow cytometric measurements are used to classify beads within an exposed bead set to determine the presence of identical or nonidentical sequences within the test sample. The inventive technology enables the rapid analysis of DNA sequences and detection of point mutations, deletions and/or inversions while also reducing the cost and time for performing genetic assays.

Claims

1. A method for flow cytometric analysis to detect a genetic mutation in a PCR product amplilied from a nucleic acid sample containing a target gene of interest, said method comprising the steps of:

(a) obtaining beads coupled to an oligonucleotide molecule, said oligonucleotlde molecule including a polymorphic site, said polymorphic site including said genetic mutation or the wild type sequence found at the analogous position of said genetic mutation in a wild type target gene;

(b) forming a mixture by mixing said beads, said PCR product, and a fluorescently labeled nucleic acid probe, said fluorescently labeled nucleic acid probe being;

(i) complementary to said oligonucleotide molecule; and

(ii) capable of being competitively inhibited from binding to said oligonucleotide molecule by said PCR product;

(c) forming a reacted sample by incubating said mixture under conditions wherein if said PCR product includes said polymorphic site, said PCR product competitively inhibits binding of said fluorescently labeled nueleic acid probe to said oligonucleotide molecule;

(d) analyzing said reacted sample by flow cytometry to determine the fluorescence of each bead analyzed; and

(e) detecting the genetic mutation or absence thereof as a result of the degree of fluorescence on the beads.

3. The method of claim 1 or 2 wherein said genetic mutation is selected from the group consisting of mutations in multiple endocrine neoplasia type 2a (MEN2a), multiple endocrine neoplasia type 2b (MEN2b), multiple endocrine neoplasia type 1 (MEN 1), ret proto-oncogene, low density lipoprotein (LDL) receptor, neurofibromatosis type 1 (NF1), neurofibromatosis type 2 (NF type 2), breast and ovarian cancer susceptibility type 1 (BRCA1), breast and ovarian cancer susceptibility type 2 (BRCA2), breast and ovarian cancer susceptibility type 3 (BRCA3), adenomatous polyposis coli (APC), adenosine deaminase, Xeroderma Pigmentosum group A correcting (XPAC), excision repair cross complementing rodent repair deficiency complementation group 6 (ERCC6), fragile X mental retardation protein 1 (fmr1), Duchenne muscular dystrophy gene, myotonic dystrophy protein kinase, androgen receptor, Huntington'"'"'s, hypoxanthine-guanine phosphoribotransferase (HPRT), apolipoprotein E, beta-hexosaminidase alpha chain (HEX A), steroid 2-hydroxyluse, angiotensin, human nodular mixed lymphocytic and histiocytic cell mismatch repair (hNMLH1, 2), retinoblastoma susceptibility (Rb), transformation-associated protein 53 (p53), breakpoint cluster region/tyrosine-protein kinase (bcr/abl), B-cell leukemia/lymphoma 2 (bcl-2), and genes encoding ion transporters.

5. The method of claim 1 or 2 wherein said PCR primers effect amplification of a region of said target nucleic acid sequence identical in length to said oligonueleotide probe in a PCR process.

9. The method of claims 1 or 2 wherein said genetic mutation is a translocation or transposition.

10. The method of claim 9, wherein said transposition is the breakpoint cluster region/tyrosine-protein kinase (bcr/abl) chromosomes 11 to 14 gene transposition.

11. The method of claim 9, wherein said transposition is the B-cell leukemia/lymphoma 2 (bcl-2) chromosomes 15 to 17 gene transposition.

2. A method of detecting a genetic mutation in a PCR product amplified from a nucleic acid sample containing a target gene of interest, said method comprising the steps of:

(a) selecting an oligonucleotide probe, said oligonucleotide probe including a polymorphic site, said polymorphic site including said genetic mutation or the wild type sequence found at the analogous position of said genetic mutation in a wild type target gene;

(b) preparing a fluorescent nueleic acid probe complementary to said oligonucleotide probe;

(c) coupling said oligonucleotide probe to cash one of a plurality of beads to form a bead aliquot;

(d) selecting PCR primers to amplify a region of said target gene including said polymorphic site;

(e) amplifying said region of said target gene by PCR to form PCR products;

(f) mixing said bead aliquot, said PCR products and said fluorescent nucleic acid probe to form a mixture;

(g) incubating said mixture under competitive hybridization conditions, wherein if said PCR products include said polymorphic site, said PCR products competitively inhibit binding of said fluorescent nucleic acid probe to said oligonucleotide probe;

(h) measuring the fluorescence of said beads by flow cytometry; and

(i) detecting said genetic mutation, or absence thereof, as a function of the measured fluorescence of said beads.

4. The method of claim 2 wherein said oligonucleotide probe has a length of between 5 and 500 nucleotides.

6. A kit for detection of a genetic mutation in a sample of nueleic acid, said kit comprising:

(a) a first container containing beads coupled to an oligonucleotide capable of specifically hybridizing with a selected PCR product of interest;

(b) a second container containing a PCR primer which effects amplification of a section of nucleic acid complementary to said oligonucleotide in a PCR process; and

(c) a third container containing a fluorescent labeled nucleic acid probe which selectively hybridizes to said oligonucleotide.

7. The kit of claim 6, wherein said genetic mutation is selected from the group consisting of mutations in multiple endocrine neoplasia type 2a (MEN2a), multiple endocrine neoplasia type 2b (MEN2b), multiple endocrine neoplasia type 1 (MEN1), ret proto-oncogene, low density lipoprotein (LDL) receptor, neurofibromatosis type 1 (NF1), neurofibromatosis type 2 (NF type 2), breast and ovarian cancer susceptibility type 1 (BRCA1), breast and ovarian cancer susceptibility type 2 (BRCA2), breast and ovarian cancer susceptibilky type 3 (BRCA3), adenomatous polyposis coli (APC), adenosine deaminase, Xeroderma Pigmentosum group A correcting (XPAC), excision repair cross complementing rodent repair deficiency complementation group 6 (ERCC6), fragile X mental retardation protein 1 (fmr1), Duchenne muscular dystrophy gene, myotionic dystrophy protein kinase, androgen receptor, Huntington'"'"'s, hypoxanthine-guanine phosphoribotransferase (HPRT), apolipoprotein E, beta-hexosaminidase alpha chain (HEXA), steroid 2-hydroxylase, angiotensin, human nodular mixed lymphocytic and histiocytic cell mismatch repair (hNMLH1, 2), retinoblastoma susceptibility (Rb), transformation-associated protein 53 (p53), breakpoint cluster region/tyrosine-protein kinase (bcr/abl), B-cell leukemia/lymphoma 2 (bcl-2), and genes encoding ion transporters.

8. The kit of claim 6 wherein said fluorescent labeled nucleic acid probe has a length of between 5 and 500 nucleotides.

12. The kit of claim 6, wherein said genetic mutation is a translocation or transposition.

13. The kit of claim 12, wherein said transposition is the breakpoint cluster region/tryrosine-protein kinase (bcr/abl) chromosomes 11 to 14 gene transposition.

14. The kit of claim 12, wherein said transposition is the B-cell leukemia/lymphoma 2 (bcl-2) chromosomes 15 to 17 gene transposition.

15. A kit for detection of a genetic mutation in a sample of nucleic acid, said kit comprising:

(a) a first container containing beads coupled to an oligonucleotide capable of specifically hybridizing with a selected PCR product of interest; and

(b) a second container containing a fluorescent labeled nucleic acid probe which is fully complementary to said oligonucleotide.

16. The kit of claim 15, wherein said genetic mutation is selected from the group consisting of mutations in multiple endocrine neoplasia type 2a (MEN2a), multiple endocrine neoplasia type 2b (MEN2b), multiple endocrine neoplasia type 1 (MEN 1 ), ret proto-oncogene, low density lipoprotein (LDL) receptor, neurofibromatosis type 1 (NF 1), neurofibromatosis type 2 (NF type 2), breast and ovarian cancer susceptibility type 1 (BRCA1), breast and ovarian cancer susceptibility type 2 (BRCA2), breast and ovarian cancer susceptibility type 3 (BRCA3), adenomatous polyposis coli (APC), adenosine deaminase, Xeroderma Pigmentosum group A correcting (XPAC), excision repair cross complementing rodent repair deficiency complementation group 6 (ERCC6), fragile X mental retardation protein 1 (fmr1), Duchenne muscular dystrophy gene, myotionic dystrophy protein kinase, androgen receptor, Huntington'"'"'s, hypoxanthine-guanine phosphoribotransferase (HPRT), apolipoprotein E, beta-hexosaminidase alpha chain (HEXA), steroid 2-hydroxylase, angiotensin, human nodular mixed lymphocytic and histiocytic cell mismatch repair (hNMLH1,2), retinoblastoma susceptibility (Rb), transformation-associated protein 53 (p53), transformation-associated protein 53 (p53), breakpoint cluster region/tyrosine-protein kinase (bcr/abl), B-cell leukemia/lymphoma 2 (bcl-2), and genes encoding ion transporters.

17. The kit of claim 15, wherein said fluorescent labeled nucleic acid probe has a length of between 5 and 500 nucleotides.

18. The kit of claim 15, wherein said genetic mutation is a translocation or transposition.

19. The kit of claim 18, wherein said transposition is the breakpoint cluster region/tyrosine-protein kinase (bcr/abl) chromosomes 11 to 14 gene transposition.

20. The kit of claim 18, wherein said transposition is the B-cell leukemia/lymphoma 2 (bcl-2) chromosomes 15 to 17 gene transposition.