Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
First Claim
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1. A method of determining the sequence of a target nucleic acid sequence in a sample, comprising the steps of:
- (a) providing a solid phase comprising particles having transponders, the particles having an oligonucleotide probe attached to a surface of the solid phase particles, the transponders having memory elements and an index number indicating sequence of the probe encoded on the transponders;
(c) contacting the solid phase with a sample to form a sample mixture;
(d) denaturing nucleic acids in the sample mixture;
(e) hybridizing the nucleic acids in the sample mixture, whereby target nucleic acid sequences hybridize to complementary probes;
(f) analyzing the solid phase to detect the presence of a label indicative of binding target nucleic acid to probes;
(g) decoding the data encoded on transponders using the dedicated read/write scanner to identify the sequence of the probes to which target nucleic acids are bound.
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Abstract
A method is described for determining the sequence of nucleic acids. The method employs small solid phase particles having transponders, with a primary layer of an oligonucleotide of known sequence attached to the outer surface of the particle. A read/write scanner device is used to encode and decode data on the transponder. The stored data includes the sequence of the oligonucleotide immobilized on the transponder. The sequence of sample nucleic acids is determined by detecting annealing to an oligonucleotide bound to a particle, followed by decoding the transponder to determine the sequence of the oligonucleotide.
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Citations
11 Claims
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1. A method of determining the sequence of a target nucleic acid sequence in a sample, comprising the steps of:
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(a) providing a solid phase comprising particles having transponders, the particles having an oligonucleotide probe attached to a surface of the solid phase particles, the transponders having memory elements and an index number indicating sequence of the probe encoded on the transponders; (c) contacting the solid phase with a sample to form a sample mixture; (d) denaturing nucleic acids in the sample mixture; (e) hybridizing the nucleic acids in the sample mixture, whereby target nucleic acid sequences hybridize to complementary probes; (f) analyzing the solid phase to detect the presence of a label indicative of binding target nucleic acid to probes; (g) decoding the data encoded on transponders using the dedicated read/write scanner to identify the sequence of the probes to which target nucleic acids are bound. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of determining the sequence of target nucleic acid thought to contain a plurality of subsequences, comprising the steps of:
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(a) introducing into the sample at least two populations of solid phase particles, each particle having a transponder and having an oligonucleotide probe corresponding to one of the subsequences attached to its surface, a first population having a different oligonucleotide probe sequence than a second population and the transponders in the first population being encoded with a different identification than the transponders of the second population; (b) denaturing the nucleic acids in the sample; (c) hybridizing the nucleic acids in the sample, whereby target nucleic acid sequences hybridize to the oligonucleotide probes; (c) analyzing the particles to detect a label indicating that target nucleic acid has bound to the probe; and (d) decoding the transponder to determine the sequence of the probe. - View Dependent Claims (8, 9, 10, 11)
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Specification