Detection of organic compounds through regulation of antibody-catalyzed reactions
First Claim
1. A method for determining the presence or absence of a target organic molecule in a sample which comprises:
- a. adding a substrate molecule to the sample,b. adding a catalytic monoclonal antibody to the sample which(i) recognizes and binds the target organic molecule when the target organic molecule is present in the sample, or(ii) recognizes, binds, and transforms the substrate molecule when the target organic molecule is not present in the sample; and
c. detecting a change in the sample signifying the absence of the target organic molecule, the change being the production of a reporter molecule by an antibody-catalyzed reaction of the substrate molecule and thereby determining the presence or absence of the target organic molecule in the sample.
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Abstract
The present invention provides a method for determining the presence of a target organic molecule in a sample which comprises either (A): first, adding a substrate molecule to the sample, the substrate molecule being a conjugate of the target organic molecule and a reporter molecule; second, adding a catalytic monoclonal antibody to the sample which (1) recognizes and binds the target organic molecule when the target organic molecule is present in the sample and (2) recognizes and binds the substrate molecule when the target organic molecule is not present in the sample; and third detecting a change in the sample signifying the absence of the target organic molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of an organic molecule in the sample; or (B): first adding a substrate molecule to the sample, the substrate molecule being a conjugate of a molecule complementary to the target molecule and a reporter molecule; second adding a catalytic monoclonal antibody to the sample which (1) recognizes and binds the target organic molecule when the target molecule is present in the sample and (2) recognizes and binds the substrate molecule only if the target molecule is present in the sample; and third detecting a change in the sample signifying the presence of the target molecule, the change being the product of an antibody-catalyzed reaction and thereby determining the presence of the target organic molecule in the sample.
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Citations
25 Claims
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1. A method for determining the presence or absence of a target organic molecule in a sample which comprises:
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a. adding a substrate molecule to the sample, b. adding a catalytic monoclonal antibody to the sample which (i) recognizes and binds the target organic molecule when the target organic molecule is present in the sample, or (ii) recognizes, binds, and transforms the substrate molecule when the target organic molecule is not present in the sample; and c. detecting a change in the sample signifying the absence of the target organic molecule, the change being the production of a reporter molecule by an antibody-catalyzed reaction of the substrate molecule and thereby determining the presence or absence of the target organic molecule in the sample. - View Dependent Claims (3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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2. A method for determining the presence of a target organic molecule in a sample which comprises:
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a. adding a catalytic monoclonal antibody to the sample which (i) recognizes and binds the target organic molecule when the target organic molecule is present in the sample and (ii) recognizes, binds and transforms a substrate molecule when the target organic molecule is present in the sample; b. adding a substrate which is the conjugate of a reporter molecule and a molecule which complements the target organic molecule through non-covalent bonding interactions; c. detecting a change in the sample signifying the presence of the target organic molecule, the change being the production of the reporter molecule by an antibody-catalyzed reaction of substrate molecule and thereby determining the presence of target organic molecule in the sample.
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Specification