Transglutaminase originating from Crassostrea gigas
First Claim
1. A transglutaminase enzyme originating from Crassostrea gigas having the following characteristics:
- a) substrate specificity in which said enzyme catalyzes the transfer of an acyl group between primary amines and γ
-carboxyamide groups of accessible glutamine residues present in a protein or a polypeptide chain;
b) molecular weight as determined by SDS-PAGE is about 83,000 to 95,000 Da;
c) optimal temperature range of 30°
C. to 50°
;
d) optimal pH range of about 7.5 to 9.5;
e) activation by calcium ions with further enhancement of activity by NaCl or KCl; and
f) inhibition by N-ethylmaleimide, monoiodo acetic acid, p-chloromercuribenzoic acid or Cu2+.
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Abstract
The present invention relates to a transglutaminase originating from Crassostrea gigas, a gene coding for the transglutaminase, a plasmid carrying the gene, a microorganism transformed with the plasmid, a method for producing an intended transglutaminase by cultivating the microorganism and a method for gelating a protein using the transglutaminase originating from Crassostrea gigas. When comparing with other transglutaminases, the transglutaminase originating from Crassostrea gigas has novel characteristic properties such that it can be activated by the action of calcium ions and that it is further activated by the addition of sodium chloride and/or potassium chloride and it is of utility value, in particular, as a gelling agent for foods.
36 Citations
22 Claims
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1. A transglutaminase enzyme originating from Crassostrea gigas having the following characteristics:
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a) substrate specificity in which said enzyme catalyzes the transfer of an acyl group between primary amines and γ
-carboxyamide groups of accessible glutamine residues present in a protein or a polypeptide chain;b) molecular weight as determined by SDS-PAGE is about 83,000 to 95,000 Da; c) optimal temperature range of 30°
C. to 50°
;d) optimal pH range of about 7.5 to 9.5; e) activation by calcium ions with further enhancement of activity by NaCl or KCl; and f) inhibition by N-ethylmaleimide, monoiodo acetic acid, p-chloromercuribenzoic acid or Cu2+. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 18, 19)
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14. A gelling agent comprising a transglutaminase originating from Crassostrea gigas.
- 17. A method for gelating a protein comprising treating a protein solution or slurry having a concentration of not less than 0.1% by weight with a transglutaminase originating from Crassostrea gigas for a time sufficient to convert the solution or slurry into a gel.
Specification