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Method for producing transgenic cereal plants

  • US 5,736,369 A
  • Filed: 06/07/1995
  • Issued: 04/07/1998
  • Est. Priority Date: 07/29/1994
  • Status: Expired due to Term
First Claim
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1. A method for producing transgenic cereal plants that will transmit introduced DNA to progeny, comprising the steps of(A) introducing foreign DNA into target cells selected from the group consisting of (i) cells of a meristem that is not enclosed by primordial sheathing leaves and (ii) cells that contribute to said meristem;

  • then(B) inducing reorganization of said meristem to increase transgenic sector size, whereby the likelihood that a transgenic sector will contribute to germline transmission is increased, wherein said reorganization is effected through at least one manipulation selected from the group consisting of (i) imposition of a nonlethal selective pressure on said meristem, (ii) mechanically-induced meristem reorganization, and (iii) hormonally-induced shoot multiplication combined with nonlethal selective pressure; and

    thereafter(C) exposing said meristem to conditions under which it differentiates to form a plantlet, wherein said plantlet contains said transgenic sector or is homogeneously transformed by said foreign DNA, such that said plantlet can be grown into a transformed cereal plant that will transmit said foreign DNA to progeny.

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