Mutant A-amylase

• US 5,736,499 A
• Filed: 06/06/1995
• Issued: 04/07/1998
• Est. Priority Date: 06/06/1995
• Status: Expired due to Term

• Current Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Original Assignee: Genencor International Incorporated (International Flavors & Fragrances Incorporated)
• Inventors: Mitchinson, Colin, Requadt, Carol Ann, Ropp, Traci Helen, Solheim, Leif P.
• Primary Examiner(s): Lieberman, Paul
• Assistant Examiner(s): Fries, Kery A.
• Application Number:
  US08/468,700
• Time in Patent Office: 1,036 Days
• Field of Search: 252/174.12, 252/DIG. 12, 435/201, 435/202, 435/203, 435/204, 510/392, 510/393, 510/530
• US Class Current: 510/392
• CPC Class Codes: C11D 3/386 Preparations containing enzymes , e.g. protease or amylase
• CPC Class Codes: C12N 9/2417 from microbiological source

Abstract

Novel α-amylase enzymes are disclosed in which one or more asparagine residues are substituted with a different amino acid or deleted. The disclosed α-amylase enzymes show altered or improved low pH starch hydrolysis performance, stability and activity profiles.

Claims

1. An α

-amylase derived from Bacillus in that it is the expression product of a mutated Bacillus DNA, sequence encoding an α

-amylase, the mutated DNA said α

-amylase comprising the deletion or substitution of a residue corresponding to N188 in Bacillus lichenformis.

2. The α

-amylase according to claim 1, wherein said α

-amylase comprises a substitution corresponding to N188S in Bacillus licheniformis.

3. The α

-amylase according to claim 1, wherein said deletion or substitution further comprises the deletion or substitution of a methionine or tryptophan residue.

4. The α

-amylase according to claim 3, wherein said deletion or substitution of said methionine or tryptophan residue comprises a substitution or deletion corresponding to M15, W138 or M197 in Bacillus lichenformis.

5. The α

-amylase according to claim 4, wherein said substitution of said methionine residual comprises a substitution corresponding to M15T, W138 or M197T in Bacillus lichenformis.

6. A DNA encoding the α

-amylase according to claim 1.

7. A DNA encoding the α

-amylase according to claim 3.

8. A DNA encoding the α

-amylase according to claim 4.

9. A DNA encoding the α

-amylase according to claim 4.

10. A DNA encoding the α

-amylase according to claim 1.

11. An expression vector comprising the DNA of claim 6.

12. An expression vector comprising the DNA of claim 7.

13. An expression vector comprising the DNA of claim 8.

14. An expression vector comprising the DNA of claim 9.

15. An expression vector comprising the DNA of claim 10.

16. A host cell transformed with the expression vector of claim 11.

17. A host cell transformed with the expression vector of claim 12.

18. A host cell transformed with the expression vector of claim 13.

19. A host cell transformed with the expression vector of claim 14.

20. A host cell transformed with the expression vector of claim 15.

21. An α

-amylase according to claim 1 having enhanced low pH performance.

22. A detergent composition comprising the α

-amylase according to claim 1.

23. The detergent composition according to claim 22, wherein said detergent is useful in laundering soiled fabric.

24. The detergent composition according to claim 22, wherein said detergent is useful in washing soiled dishes.

25. An α

-amylase derived from Bacillus comprising the substitutions corresponding to M15T/N188S in Bacillus lichenformis.