Optical biosensor matrix

• US 5,738,825 A
• Filed: 05/12/1997
• Issued: 04/14/1998
• Est. Priority Date: 07/20/1993
• Status: Expired due to Term

• Current Assignee: Unaxis Balzers AG (Oerlikon USA, Inc. (Florida))
• Original Assignee: Artificial Sensing Instruments ASI AG, Balzers Aktiengesellschaft (OC Oerlikon Corporation AG)
• Inventors: End, Peter, Legay, Francois, Pfefferkorn, Roland, Briquet, Veronique, Zetting, Alois, Tiefenthaler, Kurt, Marbach, Peter, Rudigier, Helmut
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/854,586
• Time in Patent Office: 337 Days
• Field of Search: 422/82.11, 422/102, 436/42, 436/164, 436/165
• US Class Current: 422/82.11
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 2201/0446 Multicell plate, sequential

Abstract

A detection cell which is used as a component of an optical biosensor comprises a transparent base plate and a sample plate on the base plate. The sample plate has a matrix of wells extending through it to each to receive a sample. The base plate includes a waveguiding film and a diffraction grating means to in couple an incident light field into the waveguiding film beneath a well to generate a diffracted light field to enable detection of a change in the effective refractive index of the waveguiding film.

Claims

1. A cell array plate comprising:

a microtitre sample plate having a matrix of bores extending from a first surface of said sample plate through said sample plate and to an opposite second surface of said sample plate, the bores being arranged in rows and columns;

a base plate arrangement having first and second opposite surfaces, and extending along said second opposite surface of said sample plate, said first surface of said base plate arrangement being in intimate contact with said opposite surface of said sample plate so as to seal said bores of said matrix along said opposite second surface of said sample plate, thereby closing said bores at a plurality of distinct surface areas; and

a waveguiding film arrangement at said base plate arrangement and extending along said distinct surface areas, said base plate arrangement being transparent for light from said second surface of said base plate arrangement to said waveguiding film arrangement;

said base plate arrangement comprising at least one diffraction grating structure area adjacent each of said plurality of distinct surface areas for coupling light impinging through said second surface of said base plate arrangement into said waveguiding film arrangement along a distinct surface area, and from said waveguiding film arrangement adjacent said distinct surface area, back through said second surface of said base plate arrangement;

said base plate arrangement with said waveguiding film arrangement and said diffraction grating structure areas, substantially preventing light coupled into said waveguiding film arrangement at one of said plurality of distinct surface areas, from propagating into the waveguiding film arrangement at another one of said distinct surface areas neighboring said one of said distinct surface areas, and thereby substantially preventing cross-talk between said distinct surface areas.

2. The plate of claim 1, wherein said waveguiding film arrangement comprises at least two distinct diffraction grating structure areas at each of said distinct surface areas.

3. The plate of claim 1, wherein said waveguiding film arrangement comprises first diffraction gratings and second diffraction gratings, said first and second diffraction gratings being arranged in a rectangular pattern with respect to each other.

4. The plate of claim 1, including an optical coupling layer extending along each of said distinct surface areas.

5. The plate of claim 1, wherein said waveguiding film arrangement is made of titanium dioxide.

6. The plate of claim 1, wherein said waveguiding film arrangement is made of a material comprising at least one of the oxides Ta₂ O₅, HfO₂, Si₃ N₄, ZrO₂, niobium oxide, Al₂ O₃ or a mixture of SiO₂ and TiO₂ or at least one of the oxinitrides HfON or SiON.

7. The plate of claim 1, wherein said base plate arrangement is removably connected to said sample plate.

8. The plate of claim 1, comprising at least three distinct diffraction gratings at each of said distinct surface areas.

9. The plate of claim 1, wherein said waveguiding film arrangement comprises a continuous waveguiding film.

10. The plate of claim 9, wherein a continuous diffraction grating structure area is provided along said base plate arrangement, forming said at least one diffraction grating structure area at each of said distinct surface areas.

11. The plate of claim 1, wherein said base plate arrangement includes a base plate, said waveguiding film arrangement being deposited on said base plate.

12. The plate of claim 11, wherein each diffraction grating structure area is provided at an interface between said waveguiding film arrangement and said base plate.