Liposome compositions and kits therefor
First Claim
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1. A method for preparing a radio labelled liposome, the method comprising the steps of:
- (a) adding a reducing agent to a liposome under vacuum to form a treated liposome;
(b) dehydrating under vacuum said treated liposome, to form a dehydrated liposome;
rehydrating under vacuum said dehydrated liposome, to form a rehydrated liposome; and
(d) adding a radio label to said rehydrated liposome under vacuum, to form a radio labelled liposome binding at least about 90% of an added radio label.
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Abstract
A method for preparing a radio labelled liposome, the method including the steps of at least once dehydrating and rehydrating a treated liposome to form a binding liposome, and adding a radio label to said binding liposome to form the radio labelled liposome.
158 Citations
8 Claims
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1. A method for preparing a radio labelled liposome, the method comprising the steps of:
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(a) adding a reducing agent to a liposome under vacuum to form a treated liposome; (b) dehydrating under vacuum said treated liposome, to form a dehydrated liposome; rehydrating under vacuum said dehydrated liposome, to form a rehydrated liposome; and (d) adding a radio label to said rehydrated liposome under vacuum, to form a radio labelled liposome binding at least about 90% of an added radio label.
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2. A method for preparing a radio labelled liposome, the method comprising the steps of:
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(a) adding a reducing agent to a liposome under vacuum to form a treated liposome; (b) dehydrating said treated liposome under vacuum, to form a dehydrated liposome; (c) rehydrating said dehydrated liposome under vacuum, to form a rehydrated liposome; and (d) adding a radio label to said rehydrated lipsome under vacuum, to form the radio labelled liposome. - View Dependent Claims (3, 5, 6, 7, 8)
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4. A method for preparing a radio labelled liposome, the method comprising the steps of:
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(a) adding stannous chloride to a liposome under vacuum to form a treated liposome; (b) dehydrating said treated liposome under vacuum, to form a dehydrated liposome; (c) rehydrating said dehydrated liposome under vacuum, to form a rehydrated liposome; and (d) adding a radio label to said rehydrated lipsome under vacuum, to form the radio labelled liposome.
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Specification