Multiple output referencing system for evanescent wave sensor
First Claim
1. A system for measuring at least one signal from a waveguide sensor, the system comprising:
- a waveguide sensor having at least one wave propagating surface including a reactant coating immobilized thereon, the reactant coating including a first molecular species and a labeled molecular species, the first molecular species being bound to the waveguide sensor, the first molecular species being bound to the labeled molecular species, and the labeled molecular species having an excitation wavelength and an emission wavelength;
a fluorescent reference material attached to the wave guide, the fluorescent reference material having an excitation wavelength and an emission wavelength;
a source capable of emitting electromagnetic radiation at the excitation wavelength of the fluorescent reference material;
a first detector capable of detecting the emission wavelength of the fluorescent reference material; and
a coupler for transmitting electromagnetic radiation along a first path from the source to the waveguide sensor to the first detector.
2 Assignments
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Accused Products
Abstract
An evanescent wave system and method including an optical sensor for use in assaying a reference material and at least one molecular species or analyte in a test medium or test sample for diagnostic and other applicable purposes. The sensor includes a waveguide for propagating a radiation input along its length. The radiation input causes evanescent electromagnetic waves that are capable of stimulating output emissions that are indicative of a reference material and of one or more molecular species or analytes. By comparing the emission(s) indicative of the reference material to the emission indicative of the presence of the molecular species or analyte, the presence and concentration of the molecule in the sample can be determined. The reference material provides for normalization and/or calibration of the system.
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Citations
26 Claims
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1. A system for measuring at least one signal from a waveguide sensor, the system comprising:
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a waveguide sensor having at least one wave propagating surface including a reactant coating immobilized thereon, the reactant coating including a first molecular species and a labeled molecular species, the first molecular species being bound to the waveguide sensor, the first molecular species being bound to the labeled molecular species, and the labeled molecular species having an excitation wavelength and an emission wavelength; a fluorescent reference material attached to the wave guide, the fluorescent reference material having an excitation wavelength and an emission wavelength; a source capable of emitting electromagnetic radiation at the excitation wavelength of the fluorescent reference material; a first detector capable of detecting the emission wavelength of the fluorescent reference material; and a coupler for transmitting electromagnetic radiation along a first path from the source to the waveguide sensor to the first detector. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A system for measuring at least one signal from an evanescent waveguide sensor, the system comprising:
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a waveguide sensor capable of emitting first and second wavelengths; a fluorescent reference material attached to the waveguide sensor, the fluorescent reference material having an emission wavelength approximately equal to the first wavelength; a source capable of emitting electromagnetic radiation at the first and second wavelengths; a first detector capable of detecting electromagnetic radiation at the first wavelength; a second detector capable of detecting electromagnetic radiation at the second wavelength; and a coupler for transmitting electromagnetic radiation along a first path from the source to the waveguide sensor to the first detector and along a second path from the source to the waveguide sensor to the second detector. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17)
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18. A system for measuring at least one signal from a waveguide sensor, the system comprising:
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a waveguide sensor having at least one wave propagating surface including a reactant coating mobilized thereon, the reactant coating including a first molecular species and a labeling molecular species, the first molecular species being bound to the waveguide sensor and the labeling molecular species having an excitation wavelength and an emission wavelength, the labeling molecular species being bound to the first molecular species; a fluorescent reference material attached to the wave guide, the fluorescent reference material having an excitation wavelength and an emission wavelength; a source capable of emitting electromagnetic radiation at the excitation wavelength of the fluorescent reference material; a first detector capable of detecting the emission wavelength of the fluorescent reference material; and a coupler for transmitting electromagnetic radiation along a first path from the source to the waveguide sensor to the first detector. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26)
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Specification