Apparatus for preventing cross-contamination of multi-well test plates

• US 5,741,463 A
• Filed: 09/20/1995
• Issued: 04/21/1998
• Est. Priority Date: 04/19/1993
• Status: Expired due to Fees

• Current Assignee: Ashok Ramesh Sanadi
• Original Assignee: Ashok Ramesh Sanadi
• Inventors: Sanadi, Ashok Ramesh
• Primary Examiner(s): Pyon, Harold Y.
• Application Number:
  US08/530,967
• Time in Patent Office: 944 Days
• Field of Search: 422/58, 422/69, 422/101, 422/102, 422/104, 436/165, 436/177, 436/178, 436/809, 435/301, 435/311
• US Class Current: 422/527
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A multi-well plate which prevents cross-contamination of samples through the use of a resilient gasket which covers a majority of the top of the plate and is compressed by a lid having a clamp assembly. It thus provides a sealing assembly for arrays of containers of any size or shape. The gaskets may be unitary sheets with or without an array of openings corresponding to the well openings or may consist of discrete single well gaskets. A multi-tube array is also provided which can be sealed without the need of a gasket or tight-fitting caps. A multi-well plate is also disclosed in which samples can be gas-equilibrated without the risk of microbial contamination.

Claims

1. A sealed multi-well plate having an array of wells depending from a planar principal plate surface, comprising:

a base plate defining a plurality of wells in an array, said base plate having a continuous planar principal surface, said wells extending below said continuous planar principal surface;

said base plate further defining an array of well openings at said continuous planar surface of said base plate corresponding with said wells;

a unitary lid plate having an uninterrupted planar surface, wherein said uninterrupted planar surface of said lid plate has an area sufficient to extend over the majority of said well openings;

a gasket disposed between said base plate and said lid plate and overlying said well openings;

said contact between said uninterrupted planar surface of said lid plate and said continuous planar principal surface of said base plate defining with said wells closed well chambers for containing a sample;

a closure snap fitting along the perimeter of and integral with said base plate and said lid plate, wherein said closure snap fitting releasably and securely holds said lid plate to said base plate and compresses said gasket to close said well chambers.

2. The apparatus recited in claim 1 wherein said closure snap fitting includes a flexible, integral snap fit portion on said lid and an engagement surface on said base plate, wherein said flexible, integral snap fit portion of said lid engages said engagement portion of said base plate.

3. The apparatus recited in claim 1, wherein said closure snap fitting includes a plurality of projections on said base plate, a plurality of holes defined by a flange extending from the edge of said lid plate in register with said plurality of base plate projections and further including a snap fit clip for engaging said projections to secure said lid plate to said base plate.

4. The apparatus recited in claim 1, wherein said base plate comprises a tube tray holder and a plurality of tubes held in said tube tray holder, said plurality of tubes defining said plurality of wells.