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DNA cycle sequencing

  • US 5,741,640 A
  • Filed: 05/18/1995
  • Issued: 04/21/1998
  • Est. Priority Date: 09/27/1991
  • Status: Expired due to Term
First Claim
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1. A method for sequencing DNA, comprising the steps of:

  • a) contacting a polynucleotide primer complementary to a region of said DNA to be sequenced, and said DNA to be sequenced, together in the presence of a DNA polymerase, and between one and three dNTPs at least one dNTP being labeled to allow extension of said primer by addition of one or more said dNTPs to said primer, to form an extended primer,b) dissociating said extended primer from said DNA,c) repeating steps a) and b) a plurality of times, andd) contacting said extended primer with said DNA to be sequenced in the presence of a DNA polymerase, four dNTPs and a chain terminating agent.

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