Alkaline glucose oxidase obtained from cladosporium oxysporum

• US 5,741,688 A
• Filed: 05/25/1995
• Issued: 04/21/1998
• Est. Priority Date: 05/03/1994
• Status: Expired due to Fees

• Current Assignee: Novozymes A/S (Novozymes)
• Original Assignee: Novo Nordisk A/S
• Inventors: Si, Joan Qi, Oxenb.o slashed.ll, Karen M., Aagaard, Jesper
• Primary Examiner(s): Lankford, Jr., Leon B.
• Assistant Examiner(s): Tafe, Christopher R.
• Application Number:
  US08/446,645
• Time in Patent Office: 1,062 Days
• Field of Search: 435/190, 426/10, 426/20, 426/28, 426/61
• US Class Current: 435/190
• CPC Class Codes: A21D 8/042 with enzymes
• CPC Class Codes: C11D 3/38654 containing oxidase or reductase
• CPC Class Codes: C12N 9/0006 acting on CH-OH groups as donors (1.1)

Abstract

A glucose oxidase obtained from a Cladosporium oxysporum strain, designated as CBS 163.94, characterized by a pH-optimum in he range pH 6-7, having more than 75% of maximum activity at pH 8, determined at 30° C. with D-glucose as substrate.

Claims

1. An isolated glucose oxidase having a pH optimum in the range pH 6-7, determined at 30°

C. with D-glucose as substrate, having more than 75% of maximum activity at pH 8, determined at 30°

C. with D-glucose as substrate, and is obtained from a strain of Cladosporium oxysporum, designated as CBS 163.94.

2. A dough conditioner composition comprising the glucose oxidase of claim 1.

3. A pre-mix for baked products which comprise the glucose oxidase of claim 1.

4. A method for producing the glucose oxidase of claim 1 comprising culturing a cell transformed with a DNA construct comprising a DNA sequence encoding said glucose oxidase under conditions conducive to the production of the glucose oxidase and subsequently recovering the glucose oxidase from the culture.