Method of immobilizing water-soluble bioorganic compounds on a capillary-porous carrier

• US 5,741,700 A
• Filed: 06/02/1995
• Issued: 04/21/1998
• Est. Priority Date: 08/11/1993
• Status: Expired due to Term

• Current Assignee: U Chicago Argonne, LLC
• Original Assignee: University of Chicago
• Inventors: Florentiev, Vladimir Leonidovich, Ivanov, Igor Borisovich, Timofeev, Eduard Nikolaevich, Mirzabekov, Andrei Darievich, Ershov, Gennady Moiseevich
• Primary Examiner(s): Marschel, Ardin H.
• Assistant Examiner(s): Riley, Jezia
• Application Number:
  US08/411,711
• Time in Patent Office: 1,054 Days
• Field of Search: 435/6, 435/287.1, 435/287.2, 435/287.9, 536/23.1, 536/22.1, 536/25.3, 536/25.4, 530/300
• US Class Current: 435/287.1
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00646 the compounds being bound to beads immobilised on the solid supports
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/0065 by the use of liquid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/6834 Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof

Abstract

The method for immobilizing water-soluble bioorganic compounds to capillary-porous carrier comprises application of solutions of water-soluble bioorganic compounds onto a capillary-porous carrier, setting the carrier temperature equal to or below the dew point of the ambient air, keeping the carrier till appearance of water condensate and complete swelling of the carrier, whereupon the carrier surface is coated with a layer of water-immiscible nonluminescent inert oil and is allowed to stand till completion of the chemical reaction of bonding the bioorganic compounds with the carrier.

Claims

1. A method for immobilizing water-soluble bioorganic compounds on to a carrier bondable with the compounds, comprising applying water-soluble bioorganic compounds onto a bondable carrier having a matrix of cells and kept thereon until completion of the chemical bonding between said bioorganic compounds and said carrier matrix, application of water-soluble bioorganic solutions being followed by setting the temperature of the carrier equal to or below the dew point of the ambient air and the carrier is allowed to stand until water condensate swells said matrix cells, whereupon the carrier surface is coated with a layer of water-immiscible nonluminescent inert oil and allowed to stand until the reaction of bonding the bioorganic compounds with the carrier is completed.

2. A method according to claim 1 wherein the bioorganic compounds are selected from the group consisting of oligonucleotides and polynucleotides.

3. A method according to claim 1 wherein said carrier comprises polyacrylamide gel.

4. A method according to claim 1 wherein said carrier is kept under said oil layer for about 48 hours.