Detection of bound analyte by magnetic partitioning and masking

• US 5,741,714 A
• Filed: 07/16/1996
• Issued: 04/21/1998
• Est. Priority Date: 07/18/1995
• Status: Expired due to Term

• Current Assignee: Janssen Diagnostics, LLC (Johnson & Johnson)
• Original Assignee: Immunivest Corporation
• Inventors: Liberti, Paul A.
• Primary Examiner(s): WOLSKI, SUSAN C
• Application Number:
  US08/683,812
• Time in Patent Office: 644 Days
• Field of Search: 209/213, 209/214, 210/695, 210/222, 356/300, 356/306, 356/307, 422/57, 422/58, 422/68.1, 422/82.05, 422/82.08, 435/7.92, 435/7.93, 435/7.94, 435/287.2, 435/975, 435/75, 436/518, 436/526, 436/531, 436/534, 436/805, 436/807
• US Class Current: 436/526
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 977/779 Possessing nanosized particles, powders, flakes, or clusters other than simple atomic impurity doping
• CPC Class Codes: Y10S 977/883 Fluidic self-assembly, FSA
• CPC Class Codes: Y10S 977/96 Of magnetic property

Abstract

A method and apparatus for determining qualitatively or quantitatively the presence of analyte bound to a separation media without doing a bound/free separation. In the method, the bound fraction is collected in an assay region of a body of liquid which includes the free analyte, and the assay is performed by comparing the radiant-energy response in the assay region to the radiant-energy response in a control region of the body of liquid which is free of bound analyte. The apparatus has a chamber which contains the body of liquid, one or more collection elements and a control element and position in the body of liquid parallel to an opaque wall which has a colliminating slit in registry with each element. Each slit enables sensing of the radiant-energy response from the body of liquid between the slit and its associated elements.

Claims

1. An assay apparatus comprising:

a chamber having a plurality of walls opaque to radiant energy, except for at least one assay aperture and a control aperture which are transparent to radiant energy, said plurality of walls defining a container for a body of fluid having a mobile solid phase component;

at least one collection element within said chamber, said collection element having a collection area of predetermined dimensions exposed to said body of fluid, said at least one collection area disposed immediately adjacent to a wall of the chamber having said at least one assay aperture to define therebetween a limited assay region; and

at least one control element within said chamber, said control element having the same overall dimensions as said at least one collection element and having a control area exposed to said body of fluid and disposed immediately adjacent to said control aperture on a wall of the chamber to define therebetween a limited control region for comparison with said assay region,

said collection area being capable of capturing said mobile solid phase component, and said control area being incapable of capturing said mobile phase component, and

said limited assay region and said control region constituting a small fraction of said body of fluid.

2. The assay apparatus according to claim 1 wherein

said mobile solid phase component is a ferrofluid,

said collection element comprises a ferromagnetic element having a collection area comprising a limited exposed ferromagnetic surface, said ferromagnetic element having dimensions such that a magnetic gradient sufficient to attract said ferrofluid is induced on said ferromagnetic element in the presence of an external magnetic field, and

said control element comprises either a non-ferromagnetic element or a ferromagnetic element having a shielding coating covering the entire element.

3. The assay apparatus according to claim 2 wherein said at least one assay aperture and said control aperture are mutually coextensive in length and width with said collection area and said control area, respectively.

4. The assay apparatus according to claim 2 wherein said ferromagnetic element consists of a ferromagnetic wire having a shielding coating covering the element except for the collection area, and the control element consists of a ferromagnetic element having a shielding coating covering the entire element.

5. The assay apparatus according to claim 2 wherein said ferromagnetic element consists of a ferromagnetic wire having a shielding coating covering the element except for the collection area, and the control element consists of a non-ferromagnetic element having a control area.

6. The assay apparatus according to claim 1 including an absorbent material in said chamber adjacent said collection element and said control element to cause said body of fluid to migrate past said collection area and said control area.

7. The assay apparatus according to claim 1 wherein

said mobile solid phase component is a colloid coupled to biotin,

said collection area comprises avidin bound to a limited area of said collection element, and

said movement of said mobile solid phase is achieved by diffusion.

8. An assay method for determining the presence of an analyte in a sample comprising the steps of:

combining the sample with a first and second specific binding substance in a chamber of an assay apparatus to form a body of fluid to be assayed,

wherein said first specific binding substance is coupled to a mobile solid phase component and binds to said analyte, and said second specific binding substance is labeled with a detectable label and binds either to said analyte or to said first specific binding substance, and

wherein said assay apparatus comprises

a chamber having a plurality of walls opaque to radiant energy, except for at least one assay aperture and a control aperture which are transparent to radiant energy,

at least one collection element within said chamber, said collection element having a collection area of predetermined dimensions exposed to said body of fluid, said at least one collection area disposed immediately adjacent to wall of the chamber having said at least one assay aperture to define therebetween a limited assay region, and

at least one control element within said chamber, said control element having the same overall dimensions as said at least one collection element and having a control area exposed to said body of fluid and disposed immediately adjacent to said control aperture on a wall of the chamber to define therebetween a limited control region for comparison with said assay region,

said collection area being capable of capturing said mobile solid phase component, and said control area being incapable of capturing said mobile phase component, and

said limited assay region and said control region constituting a small fraction of said body of fluid;

incubating said body of fluid to produce free label and label bound to said mobile solid phase component in proportion to the amount of analyte in said sample;

producing movement of said mobile solid phase component through said assay region and said control region to provide for capture of said mobile solid phase component onto said collection area, but not onto said control area; and

determining the presence of said analyte by detecting, through said assay aperture, a first signal generated by free label in said assay region and said label bound to mobile solid phase component captured onto said collection area, and comparing said first signal with a second signal generated by free label in said control region and detected through said control aperture.

9. The assay method according to claim 8 wherein

said mobile solid phase component is a ferrofluid,

said collection element comprises a ferromagnetic element having a collection area comprising a limited exposed ferromagnetic surface, said ferromagnetic element having dimensions such that a magnetic gradient sufficient to attract said ferrofluid is induced on said ferromagnetic element in the presence of an external magnetic field,

said control element comprises either a non-ferromagnetic element or a ferromagnetic element having a shielding coating covering the entire element, and

said movement of said mobile solid phase component is produced by application of an external magnetic field.

10. The assay method according to claim 9 wherein said at least one assay aperture and said control aperture are mutually coextensive in length and width with said collection area and said control area, respectively.

11. The assay method according to claim 9 wherein said ferromagnetic element consists of a ferromagnetic wire having a shielding coating covering the element except for the collection area, and the control element consists of a ferromagnetic element having a shielding coating covering the entire element.

12. The assay method according to claim 9 wherein said ferromagnetic element consists of a ferromagnetic wire having a shielding coating covering the element except for the collection area, and the control element consists of a non-ferromagnetic element having a shielding coating covering the element except for the control area.

13. The assay method according to claim 8 including an absorbent material in said chamber adjacent said collection element and said control element to cause said body of fluid to migrate past said collection area and said control area.

14. The assay method according to claim 8 wherein

said mobile solid phase component is a colloid coupled to biotin,

said collection area comprises avidin bound to a limited area of said collection element, and

said movement of said mobile solid phase is achieved by diffusion.