Neurotrophic factor NNT-1

• US 5,741,772 A
• Filed: 02/03/1997
• Issued: 04/21/1998
• Est. Priority Date: 02/03/1997
• Status: Expired due to Term

• Current Assignee: Amgen Inc.
• Original Assignee: Amgen Inc.
• Inventors: Chang, Ming-shi
• Primary Examiner(s): Knode, Marian C.
• Assistant Examiner(s): BAKALYAR, HEATHER
• Application Number:
  US08/792,019
• Time in Patent Office: 442 Days
• Field of Search: 530/300, 530/350, 514/2
• US Class Current: 530/350
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/475 Growth factors; Growth regulators

Abstract

Disclosed are nucleic acids encoding novel neurotrophic factors, designated NNT-1. Also disclosed are amino acid sequences for NNT-1 polypeptides, methods for preparing NNT-1 polypeptides, and other related aspects.

Claims

1. An NNT-1 polypeptide, which has a biological activity of stimulating growth of motor or sympathetic neurons, selected from the group consisting of:

(a) the polypeptide of SEQ ID NO;

2;

(b) the polypeptide that is amino acids 1-198 of SEQ ID NO;

2;

(c) the polypeptide that is at least 70 percent identical to the polypeptide of (a) or (b); and

(d) a fragment of any of (a)-(c), which has said biological activity.

2. An NNT-1 polypeptide, which has a biological activity of stimulating growth of motor or sympathetic neurons, selected from the group consisting of:

(a'"'"') the polypeptide of SEQ ID NO;

5;

(b'"'"') the polypeptide that is amino acids 1-198 of SEQ ID NO;

5;

(c'"'"') the polypeptide that is at least 70 percent identical to the polypeptide of (a'"'"') or (b'"'"'); and

(d'"'"') a fragment of any of (a'"'"')-(c'"'"'), which has said biological activity.

3. An NNT-1 polypeptide, which has a biological activity of stimulating growth of motor or sympathetic neurons, that is the polypeptide of SEQ ID NO:

2 or a fragment of said polypeptide which has said biological activity.

5. The NNT-1 polypeptide of claim 3 or 4 that does not possess an amino terminal methionine.

6. The NNT-1 polypeptide of claim 3 or 4 that additionally possesses an amino terminal methionine.

4. An NNT-1 polypeptide, which has a biological activity of stimulating growth of motor or sympathetic neurons, that is the polypeptide of SEQ ID NO:

5 or a fragment of said polypeptide which has said biological activity.