Solvent extraction methods for delipidating plasma
First Claim
1. A method for delipidating plasma comprising subjecting the plasma to a solvent extraction step and a de-emulsification step,the solvent extraction step being carried out in a first container containing extracting solvent, the first container having a spinning container associated therewith, the solvent extraction step including the steps of passing the plasma into the spinning container, dispersing the plasma into fine droplets by spinning the spinning container, and passing the fine droplets through the extracting solvent to produce delipidated plasma,the de-emulsification step being carried out in a second container, the second container having an inlet and a homogenizer and containing a de-emulsifying medium, the de-emulsification step including the steps of passing the delipidated plasma into the second container through the inlet and homogenizing the delipidated plasma and the de-emulsifying medium.
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Abstract
A separating device for extracting cholesterol from plasma uses a spinner to disperse plasma into an extracting solvent in the form of fine droplets to improve separation efficiency, thereby making it suitable for delipidating blood plasma. Blood plasma is delipidated by providing the plasma to the spinner and dispersing the plasma into the extracting solvent in fine droplets. A de-emulsification step removes residual solvent from the plasma. Blood is removed from an animal and the blood plasma is delipidated. Delipidated plasma is de-emulsified and combined with the animal blood, which is then reintroduced into the animal.
93 Citations
12 Claims
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1. A method for delipidating plasma comprising subjecting the plasma to a solvent extraction step and a de-emulsification step,
the solvent extraction step being carried out in a first container containing extracting solvent, the first container having a spinning container associated therewith, the solvent extraction step including the steps of passing the plasma into the spinning container, dispersing the plasma into fine droplets by spinning the spinning container, and passing the fine droplets through the extracting solvent to produce delipidated plasma, the de-emulsification step being carried out in a second container, the second container having an inlet and a homogenizer and containing a de-emulsifying medium, the de-emulsification step including the steps of passing the delipidated plasma into the second container through the inlet and homogenizing the delipidated plasma and the de-emulsifying medium.
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12. A method for clinically reducing cholesterol in blood of an animal, the method comprising extracting the blood from the animal, subjecting plasma of the blood to solvent extraction to produce delipidated plasma, subjecting the delipidated plasma to a de-emulsification step to produce de-emulsified plasma, recombining the de-emulsified plasma with the removed blood to produce blood having reduced cholesterol, and reintroducing the blood having reduced cholesterol into the animal,
the solvent extraction step being carried out in a first container containing extracting solvent, the first container having a spinning container associated therewith, the solvent extraction step including the steps of passing the plasma into the spinning container, dispersing the plasma into fine droplets by spinning the spinning container, and passing the fine droplets through the extracting solvent to produce delipidated plasma, and the de-emulsification step being carried out in a second container, the second container having an inlet and a homogenizer and containing a de-emulsifying medium, the de-emulsification step including the steps of passing the delipidated plasma into the second container through the inlet and homogenizing the delipidated plasma and the de-emulsification step including the steps of passing the delipidated plasma into the second container through the inlet and homogenizing the delipidated plasma and the de-emulsifying medium to produce de-emulsified plasma.
Specification