Apparatus for transfer of biological fluids

• US 5,744,099 A
• Filed: 09/15/1995
• Issued: 04/28/1998
• Est. Priority Date: 09/15/1995
• Status: Expired due to Fees

• Current Assignee: Cytek Development, Inc. (Cytek Biosciences Inc)
• Original Assignee: Cytek Development, Inc. (Cytek Biosciences Inc)
• Inventors: Schulte, Harvey L., Chase, Eric S.
• Primary Examiner(s): Le, Long V.
• Application Number:
  US08/528,847
• Time in Patent Office: 956 Days
• Field of Search: 422/63, 422/65, 422/67, 422/73, 422/81, 422/100, 422/104, 422/82, 436/43, 436/47, 436/49, 436/52, 436/53, 436/54, 436/174, 436/179, 436/180, 73/864.01, 73/864.24, 73/864.25, 73/846.34, 141/2, 141/9, 141/263
• US Class Current: 422/82
• CPC Class Codes: G01N 2035/1032 Dilution or aliquotting
• CPC Class Codes: G01N 35/1079 with means for piercing stoppers or septums
• CPC Class Codes: Y10T 436/118339 with formation of a segmented stream
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An apparatus and method is disclosed for transferring biological fluids such as blood and serum from closed containers to multiple receiving containers. The disclosed methods and apparatus perform the transfer of an accurate and precise amount of biological liquid without dilution of the biological liquid, and without splattering of the biological liquid on the walls of the receiving container. Furthermore, the method discloses how to obtain equal concentrations of white blood cells in multiple receiving containers characteristic of their concentration in the sealed container.

Claims

1. An apparatus for undiluted aspirating and dispensing of liquids, comprising:

a conduit system including an aspiration path and a dispense path, said aspiration path including a portion of said dispense path;

means for cleaning and drying said aspiration and dispense paths prior to aspiration of said liquid;

aspirating means for undiluted aspirating said liquid from a sample vessel into and along said aspiration path including said dispense path portion of said aspiration path, said paths being cleaned and dried prior to said aspiration of said liquid;

dispensing means for undiluted dispensing said liquid along and out of said portion of said dispense path and into at least one receiving vessel; and

gaseous pushing means intersecting said dispense path at one end thereof and for generating a gaseous interface at said dispense end and pushing said liquid in front of said gaseous interface along and out of said dispense path and into said at least one receiving vessel.

2. The apparatus for undiluted aspirating and dispensing of liquids according to claim 1 further including:

a first Y junction of first, second and third conduits;

and wherein said aspirating means includes a means for filling said first and third conduits with said liquid in an undiluted state;

and further wherein said dispensing means includes means for filling said second conduit with a pilot fluid;

and further wherein said gaseous pushing means includes means for providing a gaseous interface between said liquid and said pilot fluid at said first Y junction.

3. The apparatus for undiluted aspirating and dispensing of liquids according to claim 2 further including a second Y junction of fourth, fifth and sixth conduits wherein said third and sixth conduits are disposed in fluid communication to provide both an aspirating and a dispensing path to said third conduit of said first Y junction.

4. The apparatus for undiluted aspirating and dispensing of liquids according to claim 3, further including first, second, third and forth valve means disposed on said first, second, forth and fifth conduits respectively, to provide for opening and closing said conduits to control the flow of said liquid, said pilot fluid and said gaseous interface within said first and second Y junctions and said first through sixth conduits.

5. The apparatus for undiluted aspirating and dispensing of liquids according to claim 4 further including a light refractive and transmissive fluid sensing means for sensing fluids within said first conduit;

said sensing means, said first, second third and forth valve means, and said first, third, forth, and sixth conduits cooperating to provide isolation and storage means for isolating and storing a predetermined amount of said liquid and isolating it from contamination and dilution by means of said gaseous interface between said liquid and said pilot fluid at said first Y junction.

6. The apparatus for undiluted aspirating and dispensing of liquids according to claim 5 wherein each of the aspirating, dispensing and storing means include a liquid path, and wherein each of said aspirating and dispensing means include a probe means and a vacuum generating means in vacuum communication therewith disposed at a terminating end of each of said liquid paths and further including liquid path preparation means for cleaning and drying all of said aspirating, dispensing and storing means liquid paths by the application of vacuum along said liquid paths to at first flush said liquid paths with pilot fluid, then clean and dry said liquid paths by causing air to flow therethrough, said first, second, third and fourth valve means, and said first, second, third, fourth, fifth and sixth conduits cooperating to provide said application of vacuum, air and pilot fluid flush through said liquid paths.

7. The apparatus for aspirating and dispensing liquids of claim 1 wherein the dispensing means includes:

a wettable vessel and a non wettable dispense probe tip for depositing said liquid into said receiving vessel such that when said liquid is terminated and said non wettable dispense probe is separated fom the previously dispensed liquid, then the energy of the preferential association of said liquid with said wettable vessel overcomes the internal skin effect energy of the liquid and causes an air/liquid interface at the probe tip.

8. The apparatus for aspirating and dispensing liquids of claim 7 further including:

cleaning means for introducing cleaning fluid into said apparatus;

a positive displacement pump means and negative vacuum pump means for moving said liquid and said cleaning fluid throughout said apparatus;

conduit means for interconnecting said aspirating, dispensing;

isolating and storage, cleaning and pump means; and

valve means for directing said liquid and said cleaning fluid through said conduit means and said aspirating, dispensing, isolating and storage means,

wherein said isolation and storage means for storing a predetermined amount of said liquid and isolating it from contamination by means of said gaseous interface includes a liquid sensor disposed at a first predetermined position in said conduit means, and wherein said pump means and valve means cooperate to provide said gaseous interface at a second predetermined position in said conduit so as to isolate said predetermined amount of liquid.

9. The apparatus for aspirating and dispensing liquids of claim 8 wherein said sensor includes a light sensitive means capable of distinguishing air, serum and blood based on their transmitting and refracting of light due to their respective indices of refraction.

10. The apparatus for aspirating and dispensing liquids of claim 9 wherein said aspirating means for aspirating liquid from said sample vessel includes a piercing needle and a septum, and means for raising said piercing needle so as to pierce through said septum.

11. The apparatus for aspirating and dispensing liquids of claim 10 further including sterilizing means for at first flushing with cleaning fluid and then vacuum drying both said aspirator means and said dispensing means so as to prevent dilution and contamination of said liquids.

12. The apparatus for aspirating and dispensing liquids of claim 7 further including equivalating means for providing multiple equivalent dispensed samples of a predetermined amount of liquid by dispensing at first one half of a sample in the order sample a, sample a+1, sample a+2, sample a+3 . . . , sample a+n, and then dispensing the second half of said sample in the reverse order sample a+n, . . . sample a+3, sample a+2, sample a+1 and sample a.

13. The apparatus for aspirating and dispensing liquids of claim 12 wherein said liquids may be blood, serum, saliva, urine, and chemical liquids.

14. The apparatus for aspirating and dispensing liquids of claim 1 further including vertical axis mixing means for vortex rotating the receiving vessel about the vertical axis of said receiving vessel while maintaining the opening portion of said receiving vessel within a predetermined annular radius so that said receiving vessel is always in the proper location for the next dispense operation.

15. Apparatus for undiluted aspirating and dispensing of liquids, comprising:

a conduit system including an aspiration path and a dispense path, said aspiration path including a portion of said dispense path;

aspirating means for aspirating of liquid from a sample vessel into and along said aspiration path including said dispense path portion of said aspiration path;

dispensing means for dispensing said liquid along and out of said portion of said dispense path and into at least one receiving vessel; and

contamination prevention means for insuring that no contamination including dilution occurs within said fluid conduit paths including cleaning/drying means for cleaning and drying said fluid conduit paths prior to aspiration of said liquid and means for moving said liquid along the dispensing path without contamination or dilution.

16. The apparatus for undiluted aspirating and dispensing of liquids of claim 15, wherein said fluid conduit paths include first and second ends and wherein said cleaning/drying means includes purging means for applying a vacuum to one end of each of said fluid conduit paths while venting the other end of each of said fluid conduit paths to atmosphere for at first removal of fluids within said fluid conduit paths and then air drying of each of said fluid conduit paths.

17. The apparatus for undiluted aspirating and dispensing of liquids of claim 16 wherein said means for moving said liquid along the dispensing path without contamination or dilution includes non-dilution means for generating a gaseous interface between said liquid and a pilot fluid at a predetermined midway location to provide a pilot fluid-gaseous interface-liquid sequence at the beginning of and within said dispensing path and thereafter pushing said liquid along said dispensing path by said pilot fluid-gaseous interface-liquid sequence such that said liquid never contacts said pilot fluid.

18. The apparatus for undiluted aspirating and dispensing of liquids of claim 17 wherein said non-dilution means includes

a first Y junction of first, second and third conduits and a means for filling said first and third conduits with said liquid in an undiluted state;

and further including means for filling said second conduit with a pilot fluid and means for providing a gaseous interface between said liquid and said pilot fluid at said first Y junction.

19. The apparatus for undiluted aspirating and dispensing of liquids according to claim 18 further including a second Y junction of fourth, fifth and sixth conduits wherein said third and sixth conduits are disposed in fluid communication.