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Mesoscale devices and methods for analysis of motile cells

  • US 5,744,366 A
  • Filed: 11/14/1994
  • Issued: 04/28/1998
  • Est. Priority Date: 05/01/1992
  • Status: Expired due to Term
First Claim
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1. A method of analyzing a fluid sample having cells characterized by their motility, comprising:

  • a) providing a device comprising;

    a solid substrate having a flow system which comprises at least one elongate flow channel of mesoscale cross-sectional dimension, and a receiving well communicating with said at least one channel and defining a starting point in said channel; and

    a cover for said substrate closing said channel and having a first port in registry with said receiving well, for introducing said sample into said receiving well so as to enable the motile cells of the sample to travel from the receiving well to progress points along the at least one channel, said cover further defining a second port in registry with said at least one channel at a point therealong, said flow system being filled with a carrier fluid;

    b) introducing said sample into said receiving well;

    c) controlling the resident conditions of the combined carrier fluid and sample to assure motility of said cells in said carrier fluid;

    d) observing the cells in the flow system;

    e) collecting data based on said observation; and

    f) completing the analysis using said data.

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