Programmable standard for use in an apparatus and process for the noninvasive measurement of optically absorbing compounds

• US 5,748,308 A
• Filed: 02/02/1996
• Issued: 05/05/1998
• Est. Priority Date: 02/02/1996
• Status: Expired due to Fees

• Current Assignee: Abbott Laboratories
• Original Assignee: Abbott Laboratories
• Inventors: Heinz, Robert Eric, Lindberg, John M.
• Primary Examiner(s): Hantis, K. K.
• Application Number:
  US08/595,675
• Time in Patent Office: 823 Days
• Field of Search: 356/330-334, 356/310, 356/326, 356/328, 356/39, 356/308
• US Class Current: 356/310
• CPC Class Codes: G01J 2003/1286 Polychromator in general
• CPC Class Codes: G01J 2003/2836 Programming unit, i.e. source and date processing
• CPC Class Codes: G01J 2003/2866 Markers; Calibrating of scan
• CPC Class Codes: G01J 3/02 Details
• CPC Class Codes: G01J 3/0208 using focussing or collimating elements, e.g. lenses or mirrors; performing aberration correction
• CPC Class Codes: G01J 3/0229 using masks, aperture plates, spatial light modulators or spatial filters, e.g. reflective filters
• CPC Class Codes: G01J 3/28 Investigating the spectrum using colour filters G01J3/51
• CPC Class Codes: G01J 3/457 Correlation spectrometry, e.g. of the intensity G01J3/453 takes precedence

Abstract

The present invention provides a programmable filter for use as a standard in correlation spectrometers. Also provided is the use of the programmable standard in a process for determining the concentration of an optically absorbing compound. Also provided is a method and apparatus for noninvasively determining the concentration of an optically absorbing biological sample which incorporates the programmable standard of the invention.

Claims

1. A noninvasive process of determining the concentration of an optically absorbing compound in a biological sample comprising comparing a spectrum of light passing through the sample with a spectrum of light passing through a programmable standard and correlating the spectra to determine the concentration of the compound, said programmable standard comprising (1) a dispersive element;

(2) a spatial filter aligned to receive light from the dispersive element; and

(3) a means for recombining the light which is reflected from the spatial filter, said means for recombining the light being optically aligned to receive light from the spatial filter.

2. The process of claim 1 including the steps of:

a) illuminating the sample with a light beam;

b) splitting light leaving the sample into a first light beam and a second light beam;

c) directing the first light beam to a neutral filtering means and the second light beam to the programmable standard;

d) detecting light from the neutral filtering means and the programmable standard; and

e) correlating the detected light from the neutral filtering means and the programmable standard to the concentration of the compound.

3. The process of claim 2 wherein the first light beam and the second light beam are directed by apertures and the spatial filter includes a reflective spatial light modulator.

4. The process of claim 3 wherein the reflective spatial light modulator includes a reflective liquid crystal modulator.

5. The process of claim 2 wherein the second light beam is sequentially directed to a first means for focusing light, the dispersive element, the spatial filter which reflects light back to the dispersive element, a second means for focusing light, and the second means for detecting light.

6. The process of claim 5 wherein the spatial filter includes a reflective spatial light modulator.

7. The process of claim 6 wherein the reflective spatial light modulator includes a reflective liquid crystal modulator.

8. The process of claim 1 wherein the optically absorbing compound is glucose.

9. A noninvasive process of determining the concentration of an optically absorbing compound in a biological sample comprising comparing a spectrum of light passing through the sample with a spectrum of light passing through a programmable standard and correlating the spectra to determine the concentration of the compound, said programmable standard comprising (1) a dispersive element;

(2) a spatial filter aligned to receive light from the dispersive element; and

(3) a means for recombining the light which passes through the spatial filter, said means for recombining the light being optically aligned to receive light from the spatial filter, said process including the steps of;

a) illuminating the sample with a light beam;

b) splitting light leaving the sample into a first light beam and a second light beam;

c) directing the first light beam to a neutral filtering means and the second light beam to the programmable standard;

d) detecting light from the neutral filtering means and the programmable standard; and

e) correlating the detected light from the neutral filtering means and the programmable standard to the concentration of the compound, wherein the second light beam is sequentially directed to a first means for expanding light, a variable color filtering means, a transmissive means for modulating light, a second means for expanding light and the second means for detecting light.