Remotely programmable matrices with memories

• US 5,751,629 A
• Filed: 06/07/1995
• Issued: 05/12/1998
• Est. Priority Date: 04/25/1995
• Status: Expired due to Term

• Current Assignee: Irori Technologies Incorporated
• Original Assignee: Irori
• Inventors: Nova, Michael P., Senyei, Andrew E.
• Primary Examiner(s): Fears, Terrell W.
• Application Number:
  US08/484,504
• Time in Patent Office: 1,070 Days
• Field of Search: 365/151, 365/153
• US Class Current: 506/33
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00295 the reactor vessels having pervious side walls
• CPC Class Codes: B01J 2219/00299 Generally cylindrical reactor vessels
• CPC Class Codes: B01J 2219/00308 interchangeably mounted in racks or blocks
• CPC Class Codes: B01J 2219/00344 Caps
• CPC Class Codes: B01J 2219/00461 Beads and reaction vessel together
• CPC Class Codes: B01J 2219/00463 Directed sorting
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00502 Particles of irregular geometry
• CPC Class Codes: B01J 2219/0054 Means for coding or tagging the apparatus or the reagents
• CPC Class Codes: B01J 2219/00542 Alphanumeric characters
• CPC Class Codes: B01J 2219/00549 2-dimensional
• CPC Class Codes: B01J 2219/00551 3-dimensional
• CPC Class Codes: B01J 2219/0056 Raised or sunken areas
• CPC Class Codes: B01J 2219/00567 Transponder chips
• CPC Class Codes: B01J 2219/00569 EEPROM memory devices
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/00592 Split-and-pool, mix-and-divide processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00689 : using computers
• CPC Class Codes: B01J 2219/00695 : Synthesis control routines, e.g. using computer programs
• CPC Class Codes: B01J 2219/0072 : Organic compounds
• CPC Class Codes: B01J 2219/00722 : Nucleotides
• CPC Class Codes: B01J 2219/00725 : Peptides
• CPC Class Codes: B82Y 10/00 : Nanotechnology for information processing, storage or transmission, e.g. quantum computing or single electron logic
• CPC Class Codes: C07K 1/00 : General methods for the preparation of peptides , i.e. processes for the organic chemical preparation of peptides or proteins of any length
• CPC Class Codes: C07K 1/04 : on carriers C07K1/003, C07K1/006 take precedence
• CPC Class Codes: C07K 1/047 : Simultaneous synthesis of different peptide species; Peptide libraries
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: C40B 70/00 : Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
• CPC Class Codes: G01N 15/149 : specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2035/00782 : reprogrammmable code
• CPC Class Codes: G01N 35/00732 : Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: G01N 35/00871 : Communications between instruments or with remote terminals
• CPC Class Codes: G11C 13/0014 : comprising cells based on organic memory material
• CPC Class Codes: G11C 13/0019 : comprising bio-molecules
• CPC Class Codes: G11C 13/025 : using fullerenes, e.g. C60, or nanotubes, e.g. carbon or silicon nanotubes

Abstract

Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The data storage units are preferably non-volatile antifuse memories. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, chemical modification and other uses. Methods for electroniclly tagging molecules are biological particles and matrix support materials and immunoassays and and other methods are also provided.

Claims

1. A combination of a matrix with memory, comprising:

(A) a recording device, comprising a data storage unit programmed with information that identifies a molecule or biological particle that is proximate to the matrix;

(B) a matrix material, and

(C) the molecule, biological particle, mixture of molecules, mixture of biological particles or a mixture of molecules and biological particles in proximity to the matrix, wherein;

the device is less than about 5 mm³ in size;

the matrix material is in the form of a container used for chemical syntheses;

the recording device is in contact with the matrix or in a composition that is in contact with the matrix;

the molecules are either linked to the matrix or in a composition that is in contact with the matrix and comprise nucleic acids, oligonucleotides, nucleotides, amino acids, biopolymers, organic molecules, peptides or proteins;

the biological particles are either linked to the matrix or in a composition that is in contact with the matrix and comprise viral capsids with or without packaged nucleic acids, phage, phage capsids with or without encapsulated nucleotide acid, eukaryotic cells, prokaryotic cells or fragments thereof; and

the data storage unit is electromagnetically remotely programmable.

2. The combination of claim 1, wherein the recording device comprises:

a memory device having a non-volatile memory means for storing a plurality of data points and means for receiving a transmitted write signal so that the write signal causes a stored data point corresponding to the data signal to be stored within the non-volatile memory means.

3. The combination of claim 1, wherein the recording device further comprises an antenna for receiving transmitted electromagnetic radiation.

4. The combination of claim 1, wherein the recording device is encased in the matrix material.

5. The combination of claim 1, wherein:

the memory device comprises an optical recording medium having a plurality of unique recording locations therein; and

each of plurality of unique recording locations is uniquely addressable for storing the plurality of data points.

6. The combination of claim 5, wherein the memory device comprises a shell for enclosing the optical recording medium, the shell comprising a material that is inert with respect to the optical recording medium, and is transmissive of an optical signal.

7. The combination of claim 5, wherein the each of the plurality of unique recording locations comprises a spectral hole corresponding to a unique wavelength of light.

8. The combination of claim 1, wherein at least one surface of the device is coated with the matrix material.

9. The combination of claim 1, wherein the matrix material is continuous and is in the form of a container, which contains one or more recording device(s) impregnated in the matrix or inside the container, wherein the container is transmissive of at least a portion the electromagnetic spectrum, and the portion includes at least visible light, infrared light, radio frequencies, ultraviolet light or other light.

10. The combination of claim 3, wherein the container is a microtiter plate with a plurality of wells, in which on or more of the wells contain the recording device, or is a test tube, culture dish, vial or beaker.

11. The combination of claim 1, wherein the matrix material is selected from among a polystyrene, a cellulose, a glass, a polyacrylamide, a polysaccharide, a rubber, silicon, a plastic, sand, pumice, agarose, halogenated hydrocarbon polymers, and any polymer used as a matrix in solid phase syntheses.

12. The combination of claim 1, wherein:

the matrix particles are in the form of a container;

the container is less than about 50 ml in volume or comprises a plurality of wells, each being about 1 ml or less in volume;

the container is transmissive to electromagnetic radiation selected from radio frequencies, infrared wavelengths, ultraviolet wavelengths, microwave frequencies, visible wavelengths or laser light;

the recording device comprises a memory device having a non-volatile memory means for storing a plurality of data points and means for receiving the transmitted signal so that the write signal causes a stored data point corresponding to the data signal to be stored within the non-volatile memory means;

the recording device is about 5 mm³ in size or less;

the recording device is remotely programmable using electromagnetic radiation;

the recording device is in contact with the container; and

the molecules and/or biological particles are in a composition in the container.

13. The combination of claim 12, wherein the container is comprised of a polymeric material to which molecules or biological molecules can be bound.

14. The combination of claim 12, wherein the container is a well of a microtiter plate.

15. The combination of claim 12, wherein the container is a vial or test tube.

16. A matrix particle, comprising:

a recording device in contact with the particle;

the matrix particle is less than about 10 mm³ in size, and is comprised of a polymeric material adapted for binding molecules or biological particles;

the recording device comprises a memory device having memory means for storing a plurality of data points and means for receiving the transmitted signal so that the write signal causes a stored data point corresponding to the data signal to be stored within the non-volatile memory means;

the recording device is encased in the matrix;

the recording device is about 5 mm³ in size or less;

the recording device is remotely programmable using electromagnetic radiation; and

biological particles and/or molecules are linked to the surface of the matrix.

17. A library, comprised of a plurality of the combinations of claim 16.

18. The library of claim 17 that is a combinatorial library.

19. The library of claim 16, wherein the components of the library are molecules that are peptides, oligonucleotides, peptoids or organic molecules.

20. The library of claim 19 that is a phage display library.