Neuronal progenitor cells and uses thereof

• US 5,753,505 A
• Filed: 07/06/1995
• Issued: 05/19/1998
• Est. Priority Date: 07/06/1995
• Status: Expired due to Term

• Current Assignee: Emory University
• Original Assignee: Emory University
• Inventors: Luskin, Marla B.
• Primary Examiner(s): Chambers, Jasemine C.
• Assistant Examiner(s): HAUDA, KAREN MARIE
• Application Number:
  US08/499,093
• Time in Patent Office: 1,048 Days
• Field of Search: 435/6, 435/69.1, 435/172.3, 435/375, 424/93.21
• US Class Current: 435/375
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C12N 2510/00 Genetically modified cells
• CPC Class Codes: C12N 5/0623 Stem cells
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025

Abstract

The present invention provides an isolated cellular composition comprising greater than about 90% mammalian, non tumor-derived, neuronal progenitor cells which express a neuron-specific marker and which can give rise to progeny which can differentiate into neuronal cells. Also provided are methods of treating neuronal disorders utilizing this cellular composition.

Claims

1. A method of obtaining an isolated cellular composition comprising greater than 90% mammalian, non tumor-derived, neuronal progenitor cells which express a neuronal marker and which give rise to progeny which differentiate into neuronal cells, comprising isolating cells from the portion of a mammalian brain that is the equivalent of the anterior portion of the subventricular zone at the dorsolateral portion of the anterior-most extent of the region surrounding the ventricle of a neonatal rat brain and culturing the isolated cells in the absence of mitotic inhibitors.

2. An isolated cellular composition of mammalian, non-tumor derived, neuronal progenitor cells prepared by the method of claim 1.

3. The isolated cellular composition of neuronal progenitor cells of claim 2, wherein said mammalian, non-tumor derived, neuronal progenitor cells divide for at least two generations without being first immortalized.

4. The isolated cellular composition of neuronal progenitor cells of claim 2, wherein said mammalian, non-tumor derived, neuronal progenitor cells are rat cells.

5. The isolated cellular composition of neuronal progenitor cells of claim 2, wherein said mammalian, non-tumor derived, neuronal progenitor cells are human cells.

6. The isolated cellular composition of neuronal progenitor cells of claim 2, wherein greater than 95% of the mammalian, non-tumor derived, neuronal progenitor cells express a neuronal marker and give rise to progeny which differentiate into neuronal cells.