Interference free biosensor
First Claim
1. A method of measuring an analyte of interest in a test sample, said method comprising the steps of (1) contacting the sample with fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase to form a conjugate, wherein the conjugate of carbon and peroxidase is confined to an electrically conductive surface, and (2) detecting change in electrical signal as a quantitative or qualitative measure of the amount of analyte in the sample.
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Accused Products
Abstract
Provided are microparticle forms of carbon, carbon catalysts and carbon-containing electrically conductive materials which are covalently linked to peroxidase. The carbon:peroxidase conjugates are suitable for use as substrates in conventional electrodes. Surprisingly, the conjugates display very little sensitivity to known interfering substances and thus are suitable for use as interference free electrodes.
62 Citations
20 Claims
- 1. A method of measuring an analyte of interest in a test sample, said method comprising the steps of (1) contacting the sample with fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase to form a conjugate, wherein the conjugate of carbon and peroxidase is confined to an electrically conductive surface, and (2) detecting change in electrical signal as a quantitative or qualitative measure of the amount of analyte in the sample.
- 7. A composition comprising fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase.
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16. An electrode for the determination of an analyte in a sample comprising:
fine particles of carbon selected from the group consisting of native carbon, derivatized carbon, and carbon-containing conductive compounds, said fine particles of carbon covalently linked to peroxidase to form a conjugate, said conjugate confined to an electrically conductive surface. - View Dependent Claims (17, 18, 19, 20)
Specification