Device of dispensing micro doses of aqueous solutions of substances onto a carrier and device for carrying out said method

• US 5,756,050 A
• Filed: 05/26/1995
• Issued: 05/26/1998
• Est. Priority Date: 08/11/1993
• Status: Expired due to Term

• Current Assignee: U Chicago Argonne, LLC
• Original Assignee: University of Chicago
• Inventors: Kirillov, Evgenii Vladislavovich, Ershow, Gennady Moiseevich, Mirzabekov, Andrei Darievich
• Primary Examiner(s): Pyon, Harold Y.
• Application Number:
  US08/411,792
• Time in Patent Office: 1,096 Days
• Field of Search: 422/67, 422/92, 422/95, 422/100, 422/102, 422/104, 436/174, 436/179, 436/180, 435/287.2, 435/309.1
• US Class Current: 422/501
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 19/0093 Microreactors, e.g. miniaturised or microfabricated reactors laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027
• CPC Class Codes: B01J 2219/00387 Applications using probes
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01J 2219/00783 Laminate assemblies, i.e. the reactor comprising a stack of plates
• CPC Class Codes: B01J 2219/00822 Metal
• CPC Class Codes: B01J 2219/00828 Silicon wafers or plates
• CPC Class Codes: B01J 2219/00873 Heat exchange
• CPC Class Codes: B01J 2219/00891 Feeding or evacuation
• CPC Class Codes: B01J 2219/00961 Temperature
• CPC Class Codes: B01J 2219/00995 Mathematical modeling
• CPC Class Codes: B01J 4/02 for feeding measured , i.e. prescribed quantities of reagents
• CPC Class Codes: B01L 3/0244 using pins
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 1/10 in the liquid or fluent state burettes, pipettes B01L3/02; sampling of ground water E02D1/06; metering by volume of fluids or fluent solid material G01F11/00, G01F13/00
• CPC Class Codes: G01N 2035/1037 : Using surface tension, e.g. pins or wires
• CPC Class Codes: Y10T 436/115831 : Condition or time responsive
• CPC Class Codes: Y10T 436/119163 : with aspirator of claimed structure
• CPC Class Codes: Y10T 436/12 : Condition responsive control
• CPC Class Codes: Y10T 436/143333 : Saccharide [e.g., DNA, etc.]
• CPC Class Codes: Y10T 436/25 : including sample preparation
• CPC Class Codes: Y10T 436/25625 : Dilution
• CPC Class Codes: Y10T 436/2575 : Volumetric liquid transfer

Abstract

A device for dispensing microdoses of aqueous solutions are provided, whereby the substance is transferred by the free surface end of a rodlike transferring element; the temperature of the transferring element is maintained at essentially the dew point of the ambient air during the transfer. The device may comprise a plate-like base to which are affixed a plurality of rods; the unfixed butt ends of the rods are coplanar. The device further comprises a means for maintaining the temperature of the unfixed butt ends of the rods essentially equal to the dew point of the ambient air during transfer of the aqueous substance.

Claims

1. A device for microdispensing a volume of an aqueous solution to a carrier, comprised of a base one of the sides of which carries a plurality of rods which are held firm against said base with one of their ends, the free butt ends of said rods being coplanar, wherein said device is further provided with a means for maintaining the temperature of the free butt ends essentially equal to the dew point of the ambient air, wherein the means for maintaining the temperature of the free butt ends of the rods is a battery of thermoelectric cells shaped as a plate having equal size with the base and contacting the latter on the side opposite to that carrying the rods, both the base and the rods being made of a heat-conductive material.

2. A device according to claim 1 wherein both the rods and the base are made of metal.

3. A device according to claim 2 wherein the rod ends held firm against the plate are coated with a heat-insulator.

4. A device according to claim 1 wherein the rod ends held firm against the plate are coated with a heat-insulator.

5. A device according to claim 4 wherein each butt end has a circular surface area.

6. A device according to claim 5 wherein said radius of said circular butt end surface area is about 110 micrometers.

7. A device according to claim 4 wherein the butt rod ends are provided with a hydrophilic coating while the side rod surfaces are provided with a hydrophobic coating with respect to an aqueous solution.

8. A device according to claim 7 wherein the butt rod ends are coated with glass.

9. A device according to claim 8 wherein the side surfaces of the rods are coated with fluoroplastic.

10. A device according to claim 8 wherein the side surfaces of the rods are coated with glass and then treated with a hydrophobic material.

11. A device for microdispensing a volume of an aqueous solution to a carrier, comprised of a base one of the sides of which carries a plurality of rods which are held firm against said base with one of their ends, the free butt ends of said rods being coplanar, said device further provided with a means for maintaining the temperature of the free butt ends essentially equal to the dew point of the ambient air, wherein said means for maintaining the temperature of the free butt ends of the rods is a battery of thermoelectric cells shaped as said base and contacting said base on the side opposite to that carrying the rods, both the base and the rods being made of a heat-conductive material.

12. A device according to claim 11 wherein both the rods and the base are made of metal.

13. A device according to claim 12 wherein the rod ends held firm against the plate are coated with a heat insulator.

14. A device according to claim 11 wherein the rod ends held firm against the plate are coated with a heat-insulator.

15. A device according to claim 14 wherein the free butt ends of said rods are provided with a hydrophilic coating while the side rod surfaces are provided with a hydrophobic coating with respect to an aqueous solution.

16. A device according to claim 15 wherein the free butt ends are coated with glass.

17. A device according to claim 16 wherein the side surfaces of the rods are coated with glass and treated with a hydrophobic material.