Polysaccharides and preparation thereof
First Claim
Patent Images
1. A polysaccharide comprising (1→
- 4) bonded α
-D- glucose units represented by the following formula ##STR4## wherein R is a hydrogen atom or an acetyl group in a ratio of 7;
3 of said polysaccharide having the following properties;
(1) Nature;
a white amorphous powder, without taste and odor;
(2) Solubility;
insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, tetrachloromethane, chloroform and ether, and soluble in water, formamide, and dimethylsulfoxide;
(3) pH of aqueous solution;
neutral;
(4) Component sugar;
glucose only;
(5) Molecular weight;
500 to 10,000;
(6) Specific optical rotation;
(α
)D (24°
C.)=+112°
to +116°
;
(7) Color reaction;
positive to anthrone-sulfuric acid reaction, phenol-sulfuric acid reaction, and chromotropic acid-sulfuric acid reaction, and negative to biuret reaction, Lowry-Folin reaction, Elson-Morgan reaction, and iodo reaction;
(8) Melting point;
showing no clear melting point;
(9) IR spectrum;
absorption specific to acetyl groups; and
(10) 13 C-NMR spectrum;
exhibiting signals characteristic to α
-1,4-glucan and acetyl groups.
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Abstract
A novel polysaccharide comprising (1->4) bonded α-D-glucose units represented by the following formula ##STR1## wherein R is a hydrogen atom or an acetyl group in a ratio of 7:3 is extracted and isolated from a liquid culture medium of a microorganism belonging to the class Basidiomycetes in the presence of a plant tissue extract. About 30% by mole of hydroxyl groups at the 2,3-positions of each unit is acetylated and the polysaccharide has a molecular weight of 500 to 10,000. The polysaccharide has properties of biological response modifiers (BRM) and is useful as an immuno-enhancing agent or immuno-activator.
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Citations
7 Claims
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1. A polysaccharide comprising (1→
- 4) bonded α
-D- glucose units represented by the following formula ##STR4## wherein R is a hydrogen atom or an acetyl group in a ratio of 7;
3 of said polysaccharide having the following properties;(1) Nature;
a white amorphous powder, without taste and odor;(2) Solubility;
insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, tetrachloromethane, chloroform and ether, and soluble in water, formamide, and dimethylsulfoxide;(3) pH of aqueous solution;
neutral;(4) Component sugar;
glucose only;(5) Molecular weight;
500 to 10,000;(6) Specific optical rotation;
(α
)D (24°
C.)=+112°
to +116°
;(7) Color reaction;
positive to anthrone-sulfuric acid reaction, phenol-sulfuric acid reaction, and chromotropic acid-sulfuric acid reaction, and negative to biuret reaction, Lowry-Folin reaction, Elson-Morgan reaction, and iodo reaction;(8) Melting point;
showing no clear melting point;(9) IR spectrum;
absorption specific to acetyl groups; and(10) 13 C-NMR spectrum;
exhibiting signals characteristic to α
-1,4-glucan and acetyl groups. - View Dependent Claims (2, 3)
- 4) bonded α
-
4. A method of preparing a polysaccharide having (1→
- 4) bonded α
-D- glucose units represented by the following formula ##STR5## wherein R is a hydrogen atom or an acetyl group in a ratio of 7;
3 of said polysaccharide having the following properties;(1) Nature;
a white amorphous powder, without taste and odor;(2) Solubility;
insoluble in alcohol, acetone, hexane, benzene, ethyl acetate, tetrachloromethane, chloroform and ether, and soluble in water, formamide, and dimethylsulfoxide;(3) pH of aqueous solution;
neutral;(4) Component sugar;
glucose only;(5) Molecular weight;
500 to 10,000;(6) Specific optical rotation;
(α
)D (24°
C.)=+112°
to +116°
;(7) Color reaction;
positive to anthrone-sulfuric acid reaction, phenol-sulfuric acid reaction, and chromotropic acid-sulfuric acid reaction, and negative to biuret reaction, Lowry-Folin reaction, Elson-Morgan reaction, and iodo reaction;(8) Melting point;
showing no clear melting point;(9) IR spectrum;
absorption specific to acetyl groups; and(10) 13 C-NMR spectrum;
exhibiting signals characteristic to α
-1,4-glucan and acetyl groups, comprising the steps of;cultivating a microorganism belonging to the class Basidiomycetes in a liquid culture medium in the presence of a plant tissue extract; and extracting and isolating the polysaccharide. - View Dependent Claims (5, 6, 7)
- 4) bonded α
Specification