Biomolecular optical sensors
First Claim
1. A cell comprising an artificial molecular optical sensor, said sensor comprising a predetermined transmembrane cell surface protein comprising a post-translationally generated luminescer at a predetermined residue of said protein, wherein said protein adopts one of a plurality of different interconvertable signal-dependent conformations, whereunder said luminescer provides corresponding different luminescence, wherein said cell comprises a transgene encoding said protein and said cell expresses said protein said residue is located within about 10 residues of a transmembrane domain--extracellular domain junction of said protein.
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Abstract
The invention provides protein-based sensors which report changes at a cell surface membrane. These sensors comprise an endogenous cell surface protein having a post-translationally generated luminescer at a predetermined residue. In operation, the protein adopts one of a plurality of different interconvertable signal-dependent conformations, whereunder the luminescer provides corresponding different luminescence. The cells comprise transgenes encoding the protein sensor which is translated from such transgenes by the host cell'"'"'s machinery with natural amino acids and expressed on the cell surface. After surface expression, the cell is contacted with a luminescer generating reagent which generates a luminescer at a predetermined residue of the protein. A wide variety of luminescers and chemistries for generating the selected luminescer at the target residue of the sensor protein may be used.
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Citations
8 Claims
- 1. A cell comprising an artificial molecular optical sensor, said sensor comprising a predetermined transmembrane cell surface protein comprising a post-translationally generated luminescer at a predetermined residue of said protein, wherein said protein adopts one of a plurality of different interconvertable signal-dependent conformations, whereunder said luminescer provides corresponding different luminescence, wherein said cell comprises a transgene encoding said protein and said cell expresses said protein said residue is located within about 10 residues of a transmembrane domain--extracellular domain junction of said protein.
Specification