Lipid membrane sensors
First Claim
1. A bilayer lipid membrane sensor comprising(1) a gold recording surface,(2) a first lipid layer comprising(a) a thiolipid having two phospholipid molecules, each linked through one or more --(--O--CH2 --CH2)-- units to each end of a disulphide (--S--S--) group, said one or more --(--O--CH2 --CH2)-- units being short enough to allow the thiolipid to become anchored to the gold surface by self-assembly through the sulfur atom of the broken or intact disulfide group, but long enough to trap an aqueous layer between the gold surface and the bottom of the thiolipid layer, said thiolipid being attached to and covering from 75 to 95% of the gold surface, and(b) a phospholipid providing an unattached fluid phase at room temperature and constituting the remaining 25 to 5% of the first lipid layer,(3) a second lipid layer of phospholipid, and(4) a ligand within the lipid layers capable of binding with an analyte.
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Accused Products
Abstract
A bilayer lipid membrane (BLM) sensor comprising (1) a gold recording surface, (2) a first lipid layer which is an imperfect layer of a thiolipid which comprises the residue of two phospholipid molecules linked to each end of a disulphide (--S--S--) group, each through an oxyethylene (--O--CH2 --CH2) chain which is short enough to allow the thiolipid to become anchored to the gold surface by self-assembly, but long enough to trap an aqueous layer between the gold surface and the bottom of the thiolipid layer, said thiolipid being attached to the gold surface and said imperfect layer being completed by a phospholipid which provides an unattached fluid phase at room temperature, and (3) a second lipid layer of phospholipid. The sensor is especially useful for measuring changes in electrical resistance and/or capacity when it is prepared to contain a receptor protein and an analyte containing an agonist to the receptor is contacted with the bilayer.
103 Citations
12 Claims
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1. A bilayer lipid membrane sensor comprising
(1) a gold recording surface, (2) a first lipid layer comprising (a) a thiolipid having two phospholipid molecules, each linked through one or more --(--O--CH2 --CH2)-- units to each end of a disulphide (--S--S--) group, said one or more --(--O--CH2 --CH2)-- units being short enough to allow the thiolipid to become anchored to the gold surface by self-assembly through the sulfur atom of the broken or intact disulfide group, but long enough to trap an aqueous layer between the gold surface and the bottom of the thiolipid layer, said thiolipid being attached to and covering from 75 to 95% of the gold surface, and (b) a phospholipid providing an unattached fluid phase at room temperature and constituting the remaining 25 to 5% of the first lipid layer, (3) a second lipid layer of phospholipid, and (4) a ligand within the lipid layers capable of binding with an analyte.
Specification