Assay for cardiac troponin I
First Claim
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1. Polyclonal antibodies which bind specifically to the peptideARG-GLY-GLU-LYS-GLY-ARG-ALA-LEU-SER-THR-ARG-CYS-GLN-PRO-LEU-GLU-LEU-ALA (SEQ ID NO:
- 3);
said polyclonal antibodies having essentially no cross-reactivity with skeletal tropronin I.
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Abstract
A method for detection of cardiac troponin I is described. The method is a sandwich assay employing an antibody specific for cardiac troponin I as one binding partner and troponin C as the other binding partner for cardiac troponin I.
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4 Claims
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1. Polyclonal antibodies which bind specifically to the peptide
ARG-GLY-GLU-LYS-GLY-ARG-ALA-LEU-SER-THR-ARG-CYS-GLN-PRO-LEU-GLU-LEU-ALA (SEQ ID NO: - 3);
said polyclonal antibodies having essentially no cross-reactivity with skeletal tropronin I.
- 3);
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2. Monoclonal antibody specific for the peptide:
ARG-GLY-GLU-LYS-GLY-ARG-ALA-LEU-SER-THR-ARG-CYS-GLN-PRO-LEU-GLU-LEU-ALA (SEQ ID NO;
3).
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3. A method for preparing polyclonal antibodies specific for cardiac troponin I comprising:
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(i) contacting anti-troponin I antiserum with the peptide ARG-GLY-GLU-LYS-GLY-ARG-ALA-LEU-SER-THR-ARG-CYS-GLN-PRO-LEU-GLU-LEU-ALA (SEQ ID NO;
3), under conditions which permit antibodies specific for the peptide to bind to the solid phase;(ii) separating the solid phase from the antiserum; and (iii) thereafter eluting the bound antibodies from the solid phase. - View Dependent Claims (4)
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Specification