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Compositions for chromosome-specific staining

  • US 5,756,696 A
  • Filed: 12/23/1994
  • Issued: 05/26/1998
  • Est. Priority Date: 01/16/1986
  • Status: Expired due to Term
First Claim
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1. Nucleic acid probes for use in in situ hybridization comprising a heterogeneous mixture that contains labeled nucleic acid fragments that are substantially complementary to unique nucleic acid segments, wherein said mixture comprises blocking nucleic acid having nucleic acid fragments which are substantially complementary to repetitive segments in the labeled nucleic acid, produced by the process of:

  • (a) obtaining chromosome-specific DNA fragments;

    (b) amplifying said chromosome-specific DNA fragments;

    (c) labeling said chromosome-specific DNA fragments with a fluorescent or affinity label; and

    (d) adding sufficient blocking nucleic acid to prevent substantial binding of the labeled nucleic acid sequences to repetitive nucleic acid sequences in a target DNA.

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