Optical detection device for analytical measurement of chemical substances

• US 5,757,014 A
• Filed: 04/01/1996
• Issued: 05/26/1998
• Est. Priority Date: 04/07/1995
• Status: Expired due to Fees

• Current Assignee: Ciba-Geigy Corporation (Novartis Ag)
• Original Assignee: Novartis Corporation (Novartis Ag)
• Inventors: Barnard, Steven Mark, Bruno, Alfredo Emilio, Rouilly, Marizel
• Primary Examiner(s): Glick, Edward J.
• Application Number:
  US08/625,330
• Time in Patent Office: 785 Days
• Field of Search: 250/458.1, 250/461.1, 356/417, 356/317, 356/318
• US Class Current: 250/458.1
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B

Abstract

In the optical detection device (1), the excitation light guide (11) and the emission light guide (12) form a structural unit having the end face (14) facing the fluorescence changer (4). The end face (14) physically corresponds substantially to one of the boundary surfaces of the two light guides (11, 12). As a result, the detection device (1) can be separated in a simple manner into an optical part and a mechanical part which are in optical contact only by way of the end face (14). All of the elements in the optical light path are connected in a mechanically stable manner by means of the index-matching medium 20, as a result of which the light between the light source (10) and the photoelectric sensor (13) propagates only in media having a comparable refractive index. The light source (10) is preferably a light-emitting diode. A plurality of those detection devices can be combined to form a compact detection apparatus with which the substance (3) can be examined with regard to several constituents in one measuring operation.

Claims

1. An optical detection device for analytical measurements of a substance, comprising a sample container for the substance, a fluorescence changer, a light source for emitting an excitation light, a photoelectric sensor for sensing an emission light coming from the fluorescence changer, an excitation light guide which substantially guides the excitation light emitted by the light source in such a manner that it meets at least part of the fluorescence changer, and an emission light guide which substantially guides the light emitted by the fluorescence changer in such a manner that it meets the photoelectric sensor, at least the excitation light guide comprising a material that has a refractive index gradient substantially perpendicular to the principal direction of propagation of the light in the light guide, wherein the excitation light guide is connected to the emission light guide in such a manner that the two light guides substantially form a structural unit having an end face facing the fluorescence changer, the end face physically corresponding substantially to a boundary surface of one of the two light guides, and the end face substantially forming both an optical emergence surface for the light passing from the light source through the excitation light guide and an optical entry surface for the emission light coming from the fluorescence changer.

2. A device according to claim 1, wherein the emission light guide and the excitation light guide are cemented together by means of an index-matching medium.

3. A device according to claim 1, wherein the emission light guide and the excitation light guide are inclined relative to each other in such a manner that the principal directions of propagation of the light in the two light guides, determined by optical axes, are at an oblique angle to each other.

4. A device according to claim 1, wherein the fluorescence changer is located on a carrier having a refractive index that corresponds, to within substantially +/-20%, to the refractive indices of the two light guides, and the carrier is optically connected to the end face of the two light guides.

5. A device according to claim 1, wherein a nodal line of the structural unit formed by the excitation light guide and the emission light guide lies substantially in the region of the fluorescence changer.

6. A device according to claim 1, wherein the excitation light guide and/or the emission light guide contain at least one optical gradient index element.

7. A device according to claim 1, wherein the excitation light guide comprises at least one optical filter for selecting the wavelength range of the excitation light.

8. A device according to claim 1, wherein the emission light guide comprises at least a an optical filter for selecting the wavelength range of the light meeting the photoelectric sensor.

9. A device according to claim 1, wherein the light source is a light-emitting diode that radiates coherent or incoherent light.

10. A device according to claim 1, wherein the excitation light guide and the emission light guide are located in receiving openings in a holding body, which receiving openings are inclined one to another.

11. A device according to claim 1, wherein the emission light guide is substantially made from an index-matching medium, and optionally includes an optical filter.

12. A device according to claim 1, wherein a reference light guide having an optical axis is additionally provided which is connected to the structural unit, in such manner that the optical axes of the three light guides lie in one plane and two of the optical axes form with the third substantially the same angle, and wherein, in addition, a second photoelectric sensor is provided at a planar boundary surface of the reference light guide facing away from the end face of the structural unit.

13. An optical detection apparatus for the analytical measurement of substances, which comprises at least two optical detection devices each constructed in accordance with any one of the preceding claims.

14. An apparatus according to claim 13, wherein at least all the excitation light guides and emission light guides are located in receiving openings in a holding body, which receiving openings are inclined one to another in pairs.

15. An apparatus according to claim 13, wherein all the sample containers are formed by a common measurement chamber.

16. An apparatus according to claim 13, wherein the fluorescence changers are located on a common carrier element, the carrier element having a refractive index that corresponds, to within substantially +/-20%, to the refractive indices of the light guides and wherein the said carrier element is connected to the end faces of the light guides.

17. An apparatus according to claim 13, wherein each of the excitation/emission light guides is associated with a respective fluorescence changer which is met substantially only by the excitation light from the excitation light guide associated with it.

18. An apparatus according to claim 13, wherein the fluorescence changers are sensitive to various constituents of the substance.

19. An optical analysis apparatus for fluorescence measurements of a substance, comprising a measuring device for fluorescence examinations, an electronic power supply and control unit which provides current for the measuring device, electronic amplification and evaluation means which process signals supplied by the measuring device, and a temperature measuring and regulating device for controlling and stabilizing temperature, wherein the measuring device comprises an optical detection apparatus according to claim 13.