Electrochemical biosensor
First Claim
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1. An electrochemical sensor for the detection of an analyte in a fluid test sample which comprises:
- a) an insulating base plate;
b) an electrode layer on said base plate in operative connection with an enzyme which reacts with the analyte to produce mobile electrons; and
c) a lid of deformable material which has been embossed to provide a concave area in a portion thereof while leaving a flat surface surrounding the concave portion in such a configuration that, when mated with the base, the lid and base form a capillary space in which the enzyme is available for direct contact with fluid test sample which is drawn into the capillary space by capillary action, wherein said sensor has a polymeric layer over the underside of the lid to facilitate bonding of the lid to the base upon their being mated and to increase the hydrophilic nature of the capillary space.
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Abstract
Disclosed is an electrochemical sensor which is made up of an insulating base plate bearing an electrode on its surface which reacts with an analyte to produce mobile electrons. The base plate is mated with a lid of a deformable material which has a concave area surrounded by a flat surface so that when mated to the base plate there is formed a capillary space into which a fluid test sample can be drawn. The side of the lid facing the base is coated with a polymeric material which serves to bond the lid to the base plate and to increase the hydrophilic nature of the capillary space.
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Citations
20 Claims
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1. An electrochemical sensor for the detection of an analyte in a fluid test sample which comprises:
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a) an insulating base plate; b) an electrode layer on said base plate in operative connection with an enzyme which reacts with the analyte to produce mobile electrons; and c) a lid of deformable material which has been embossed to provide a concave area in a portion thereof while leaving a flat surface surrounding the concave portion in such a configuration that, when mated with the base, the lid and base form a capillary space in which the enzyme is available for direct contact with fluid test sample which is drawn into the capillary space by capillary action, wherein said sensor has a polymeric layer over the underside of the lid to facilitate bonding of the lid to the base upon their being mated and to increase the hydrophilic nature of the capillary space. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. A method of making an electrochemical sensor for the detection of an analyte in a fluid test sample which comprises:
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a) providing an insulating base plate which has on its surface an electrode layer in operative connection with an enzyme which reacts with the analyte to produce mobile electrons; and b) mating the base plate with a lid of a deformable material a part of which encompasses a concave area with a flat surface surrounding the concave portion in such a configuration that, when mated with the base, the lid and base form a capillary space in which the enzyme is available for direct contact with the fluid test sample in the capillary space and the lid has a polymeric layer over the underside thereof to facilitate bonding of the lid to the base and increase the hydrophilic nature of the capillary space; and c) heating the base plate and lid while applying pressure therebetween to fuse the base plate to the lid. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20)
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Specification