Microplates which prevent optical cross-talk between wells
First Claim
1. A microplate for use in assaying samples, comprising:
- a plurality of wells including at least first and second adjacent wells, each one of the plurality of wells having least one sidewall that defines an interior of the one of the plurality of wells;
a layer of light permeable material that forms a bottom of each of the first and second wells; and
at least one rib of light impermeable material extending entirely through the layer of light permeable material, the at least one rib being disposed between the first and second wells to prevent light from passing between the first and second wells through the light permeable material, the at least one rib being offset from and between the sidewalls of the adjacent first and second wells so that the at least one rib is not in-line with the at least one sidewall of any of the plurality of wells.
1 Assignment
0 Petitions
Accused Products
Abstract
A microplate and a method for manufacturing microplates. The microplate is designed to reduce optical cross-talk during the assaying of samples. The microplate comprises a plurality of wells having light permeable sidewalls, a layer of light permeable material forming a bottom of the wells, and ribs of light impermeable material extending through the layer of light permeable material. The method includes steps of inserting a plate of light permeable material into a mold cavity that includes sections shaped to form the sidewalls of the plurality of wells, injecting molten light impermeable material into the mold cavity, and cooling the light impermeable material to form the microplate with the light impermeable material forming the sidewalls of each of the plurality of wells and the plate of light permeable material forming the bottom wall of each of the plurality of wells.
67 Citations
57 Claims
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1. A microplate for use in assaying samples, comprising:
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a plurality of wells including at least first and second adjacent wells, each one of the plurality of wells having least one sidewall that defines an interior of the one of the plurality of wells; a layer of light permeable material that forms a bottom of each of the first and second wells; and at least one rib of light impermeable material extending entirely through the layer of light permeable material, the at least one rib being disposed between the first and second wells to prevent light from passing between the first and second wells through the light permeable material, the at least one rib being offset from and between the sidewalls of the adjacent first and second wells so that the at least one rib is not in-line with the at least one sidewall of any of the plurality of wells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 20)
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18. A microplate for use in assaying samples, comprising:
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a plurality of wells including at least first and second adjacent wells, each one of the plurality of wells having at least one light impermeable sidewall that defines an interior of the one of the plurality of wells; a layer of light permeable material that forms a bottom of each of the first and second wells, the layer of light permeable material having a thickness; and at least one rib of light impermeable material extending through greater than 75% of the thickness of the layer of light permeable material, the at least one rib being disposed between the first and second wells and being offset from and between the sidewalls of the adjacent first and second well so that the at least one rib is not in-line with the at least one sidewall of any of the plurality of wells. - View Dependent Claims (19)
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21. A microplate for use in assaying samples, comprising:
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a plurality of wells including at least first and second adjacent wells, each of the plurality of wells having at least one light impermeable sidewall and a light permeable bottom, the at least one sidewall of each one of the plurality of wells defining an interior of the one of the plurality of wells; and at least one rib of light impermeable material disposed between the bottoms of the first and second wells so that any plane passing through the bottom of the first well and the bottom of the second well intersects the at least one rib, the at least one rib being offset from and between the sidewalls of the adjacent first and second wells so that the at least one rib is not in-line with the at least one sidewall of any of the plurality of wells. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33)
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34. A microplate for use in assaying samples, comprising:
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a plurality of wells including first and second wells, the first well having at least one sidewall that defines an interior of the first well; a layer of light permeable material that forms a bottom of each of the plurality of wells, the layer of light permeable material including a segment of light permeable material that forms the bottom of the first well, the segment of the layer of light permeable material extending beyond the at least one sidewall of the first well to underlie at least one portion of the microplate that is not below the interior of the first well; and at least one rib of light impermeable material extending entirely through the layer of light permeable material, the at least one rib being disposed between the first and second wells to prevent light from passing between the first and second wells through the light permeable material. - View Dependent Claims (35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47)
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48. A microplate for use in assaying samples, comprising:
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a plurality of wells including at least first and second wells, each of the first and second wells including a bottom, the first well including at least one sidewall that defines an interior of the first well and a segment of light permeable material that forms the bottom of the first well, the segment of light permeable material extending beyond the at least one sidewall of the first well to underlie at least one portion of the microplate that is not below the interior of the first well; and at least one rib of light impermeable material being disposed between the bottoms of the first and second wells so that any plane passing through the bottoms of the first and second wells intersects the at least one rib. - View Dependent Claims (49, 50, 51, 52, 53, 54, 55, 56, 57)
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Specification