Two-photon and multi-photon measurement of analytes in animal and human tissues and fluids
First Claim
1. A method of measuring an analyte present in animal tissue, comprising:
- providing animal tissue containing an analyte which is two-photon-excitable at a first wavelength at which said animal tissue is substantially non-absorbing, which analyte fluoresces at a second wavelength upon being two-photon-excited at said first wavelength;
irradiating said animal tissue with radiation at said first wavelength so as to excite said analyte through absorption by said analyte of two photons of said radiation at said first wavelength, wherein excitation of said analyte results in a fluorescent emission from said analyte of radiation at said second wavelength;
detecting said emission at said second wavelength; and
determining the concentration of analyte based on the detected emission.
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Abstract
A method of measuring an analyte present in animal (e.g., human) tissue or fluids such as blood or plasma. The analyte is multi-photon excitable (e.g., two-photon excitable) at a first wavelength at which the animal tissue is substantially non-absorbing. The analyte fluoresces at a second wavelength upon being excited at the first wavelength. The animal tissue is irradiated with radiation at the first wavelength so as to excite the analyte through absorption by the analyte of two or more photons of the radiation at the first wavelength. Excitation of the analyte results in a fluorescent emission from the analyte of radiation at the second wavelength. The emission at the second wavelength is detected, and the concentration of analyte determined based on the detected emission.
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Citations
22 Claims
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1. A method of measuring an analyte present in animal tissue, comprising:
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providing animal tissue containing an analyte which is two-photon-excitable at a first wavelength at which said animal tissue is substantially non-absorbing, which analyte fluoresces at a second wavelength upon being two-photon-excited at said first wavelength; irradiating said animal tissue with radiation at said first wavelength so as to excite said analyte through absorption by said analyte of two photons of said radiation at said first wavelength, wherein excitation of said analyte results in a fluorescent emission from said analyte of radiation at said second wavelength; detecting said emission at said second wavelength; and determining the concentration of analyte based on the detected emission. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 18, 19, 20, 21, 22)
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15. A method of measuring an analyte present in animal tissue, comprising:
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providing animal tissue containing an analyte which is excitable by a plurality of photons at a first wavelength at which said animal tissue is substantially non-absorbing, which analyte fluoresces at a second wavelength upon being excited at said first wavelength; irradiating said animal tissue with radiation at said first wavelength so as to excite said analyte through absorption by said analyte of said plurality of photons of said radiation at said wavelength, wherein excitation of said analyte results in a fluorescent emission from said analyte of radiation at said second wavelength; detecting said emission at said second wavelength; and determining the concentration of analyte based on the detected emission. - View Dependent Claims (16)
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17. A method of measuring an analyte present in a material, comprising:
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providing material containing an analyte which is excitable by a plurality of photons at a plurality of wavelengths at which said material is substantially non-absorbing, which analyte fluoresces at a different wavelength upon being excited at said plurality of wavelengths; irradiating the material with radiation at said plurality of wavelengths so as to excite said analyte through absorption by said analyte of a plurality of photons of said radiation at said plurality of wavelengths, wherein excitation of said analyte results in a fluorescent emission from said analyte of radiation at said different wavelength; detecting said emission at said different wavelength; and determining the concentration of analyte based on the detected emission.
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Specification