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Two-photon and multi-photon measurement of analytes in animal and human tissues and fluids

  • US 5,759,767 A
  • Filed: 10/11/1996
  • Issued: 06/02/1998
  • Est. Priority Date: 10/11/1996
  • Status: Expired due to Term
First Claim
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1. A method of measuring an analyte present in animal tissue, comprising:

  • providing animal tissue containing an analyte which is two-photon-excitable at a first wavelength at which said animal tissue is substantially non-absorbing, which analyte fluoresces at a second wavelength upon being two-photon-excited at said first wavelength;

    irradiating said animal tissue with radiation at said first wavelength so as to excite said analyte through absorption by said analyte of two photons of said radiation at said first wavelength, wherein excitation of said analyte results in a fluorescent emission from said analyte of radiation at said second wavelength;

    detecting said emission at said second wavelength; and

    determining the concentration of analyte based on the detected emission.

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