Method of nucleic acid sequence selection

• US 5,759,778 A
• Filed: 09/08/1995
• Issued: 06/02/1998
• Est. Priority Date: 08/10/1993
• Status: Expired due to Term

• Current Assignee: Life Technologies Incorporated (Thermo Fisher Scientific Incorporated)
• Original Assignee: Life Technologies Incorporated (Thermo Fisher Scientific Incorporated)
• Inventors: Lin, Jhy-Jhu, Jessee, Joel A., Li, Wu-Bo, Gruber, Christian E.
• Primary Examiner(s): Ketter, James
• Assistant Examiner(s): Yucel, Irem
• Application Number:
  US08/525,140
• Time in Patent Office: 998 Days
• Field of Search: 435/91.1, 435/91.2, 435/172.3, 435/6, 435/7.1, 435/7.2, 435/7.5, 435/18, 536/24.3
• US Class Current: 506/4
• CPC Class Codes: C12N 15/1013 by using magnetic beads
• CPC Class Codes: C12N 15/1048 SELEX
• CPC Class Codes: C12Q 1/6809 Methods for determination or identification of nucleic acids involving differential detection
• CPC Class Codes: C12Q 1/6811 Selection methods for production or design of target specific oligonucleotides or binding molecules
• CPC Class Codes: C12Q 1/6813 Hybridisation assays
• CPC Class Codes: C12Q 1/6848 characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Abstract

The present invention provides a method for the rapid isolation and recovery of a desired target DNA or RNA molecules from a mixture or library containing such molecules. The method involves the use of biotinylated probes and enzymatic repair-cleavage to eliminate undesired library members from a sample.

Claims

1. A method for recovering a desired target nucleic acid molecule from an initial mixture or library of nucleic acid molecules containing said molecule, wherein said method comprises the steps:

(A) (1) where said initial mixture or library is composed of single-stranded nucleic acid molecules, performing step (B);

or (2) where said initial mixture or library is composed of double-stranded nucleic acid molecules treating said double-stranded nucleic acid molecules to render such molecules single-stranded, then performing step (B);

(B) incubating the single-stranded nucleic acid molecules of said mixture or library in the presence of a haptenylated nucleic acid probe molecule, said probe molecule comprising a unique nucleotide sequence complementary to a nucleotide sequence of said desired target molecule;

said incubation being under conditions sufficient to permit said probe to hybridize to said desired target molecule and to thereby generate a hybridized molecule wherein said desired target molecule is bound to said probe;

(C) capturing said hybridized molecule of step (B) by incubating said hybridized molecule in the presence of a binding ligand of the hapten of said haptenylated probe, said binding ligand being conjugated to a support;

said incubation being sufficient to permit said hybridized molecule to become bound to said binding ligand of said support;

(D) separating said bound hybridized desired target molecule from unbound nucleic acid molecules; and

(E) recovering said desired target molecule from said support.

2. The method of claim 1, wherein in step (B), said desired incubation is under conditions which minimize random hybridization.

3. The method of claim 1, wherein said desired target nucleic acid molecule is a DNA molecule.

4. The method of claim 3, wherein said initial mixture or library is composed of single-stranded DNA.

5. The method of claim 4, wherein said single-stranded DNA is circular.

6. The method of claim 4, wherein said single-stranded DNA is linear.

7. The method of claim 4, wherein the single-stranded DNA of said initial mixture or library is selected from the group consisting of single-stranded plasmids, single-stranded cosmids and single-stranded phagemids.

8. The method of claim 7, wherein the single-stranded DNA of said initial mixture or library are single-stranded plasmids.

9. The method of claim 7, wherein the single-stranded DNA of said initial mixture or library are single-stranded cosmids.

10. The method of claim 7, wherein the single-stranded DNA of said initial mixture or library are single-stranded phagemids.

11. The method of claim 3, wherein said initial mixture or library is composed of double-stranded DNA molecule.

12. The method of claim 11, wherein said double-stranded DNA is circular.

13. The method of claim 11, wherein said double-stranded DNA is linear.

14. The method of claim 11, wherein the double-stranded DNA of said initial mixture or library is selected from the group consisting of double-stranded plasmids, double-stranded cosmids and double-stranded phagemids.

15. The method of claim 14, wherein the double-stranded DNA of said initial mixture or library are double-stranded plasmids.

16. The method of claim 14, wherein the double-stranded DNA of said initial mixture or library are double-stranded cosmids.

17. The method of claim 14, wherein the double-stranded DNA of said initial mixture or library are double-stranded phagemids.

18. The method of claim 14, wherein said mixture or library of double-stranded DNA is nicked prior to said treatment of step (A)(2).

19. The method of claim 18, wherein said treatment step comprises degradation of one strand of said double stranded DNA.

20. The method of claim 1, wherein said hapten is biotin.

21. A method according to claim 20, wherein a biotin molecule is covalently bonded to the 3'"'"' terminus of said probe.

22. The method of claim 20, wherein said binding ligand of said biotin is selected from the group consisting of avidin, streptavidin, an antibody that binds biotin and an antibody fragment that binds biotin.

23. The method of claim 22, wherein said binding ligand of biotin is avidin.

24. The method of claim 22, wherein said binding ligand of biotin is streptavidin.

25. The method of claim 22, wherein said binding ligand of biotin is an antibody that binds biotin.

26. The method of claim 22, wherein said binding ligand of biotin is an antibody fragment that binds biotin.

27. The method of claim 1, wherein said separating step (D) is accomplished by magnetic means.

28. The method of claim 27, wherein said support is a paramagnetic bead.

29. The method of claim 1, which additionally includes the step:

(F) incubating said single-stranded desired target molecule of step (E) in the presence of a primer nucleic acid molecule complementary thereto;

said incubation being under conditions sufficient to permit hybridization between said primer and said desired target molecule, and further sufficient to permit the template-dependent extension of said primer to thereby generate a nucleic acid molecule complementary to said desired target molecule.

30. The method of claim 29, wherein said primer molecule of step (F) is complementary to the same sequence of said desired target molecule as said probe molecule of step (B).

31. The method of claim 29, wherein in said primer molecule of step (F) is not complementary to the same sequence of said desired target molecule as said probe molecule of step (B).

32. The method of claim 29, wherein in step (B), said probe has a degenerate sequence.

33. The method of claim 29, wherein in step (F), said primer has a degenerate sequence.

34. The method of claim 29, wherein, in step (F), a second primer is additionally provided, said second primer being complementary to a sequence of said generated nucleic acid molecule that is complementary to said desired target molecule, and wherein step (F) is conducted under conditions sufficient to permit a selective amplification of said desired target molecule.

35. The method of claim 34, wherein said selective amplification is mediated by a polymerase chain reaction.

36. The method of claim 1, which additionally comprises the step of transforming the recovered desired target molecule of step (E) into a host cell.

37. The method of claim 29, wherein said step (F) generates a double-stranded nucleic acid molecule comprising said target molecule, and wherein said method additionally comprises the step:

(G) transforming said double-stranded desired target molecule of step (F) into a host cell.

38. The method of claim 35, which additionally comprises the step of transforming the amplified desired target molecule into a host cell.

39. The method of claim 37, wherein said double standard desired target molecule is selected from the group consisting of double stranded plasmids, double stranded cosmids and double stranded phagemids.

40. A method for recovering a desired target nucleic acid molecule from a sample comprising the steps:

(A) incubating said sample in the presence of a haptenylated nucleic acid probe molecule, said probe molecule comprising a unique nucleotide sequence complementary to said desired target molecule;

said incubation being under conditions sufficient to permit said probe to hybridize to said desired target molecule and to thereby generate a hybridized molecule wherein the desired target molecule is bound to said probe;

(B) capturing said hybridized molecule of step (A) by incubating said hybridized molecule in the presence of a binding ligand of the hapten of said haptenylated probe, said binding ligand being conjugated to a support;

said incubation being, sufficient to permit said hybridized molecule to become bound to said binding ligand of said support;

(C) separating said bound hybridized desired target molecule from unbound nucleic acid molecules;

(D) incubating said desired target molecule of step (C) in the presence of a primer nucleic molecule complementary to a sequence of the desired target molecule;

said incubation being under conditions sufficient to generate a double stranded desired target molecule;

(E) releasing said double stranded target molecule from said solid support; and

(F) transforming said double stranded desired target molecule of step (D) into a host cell.

41. The method of claim 40, wherein said double stranded desired target molecule is a DNA molecule which is selected from the group consisting of a plasmid, a cosmid, and a phagemid.

42. The method of claim 41, wherein said hapten is biotin and said binding ligand is selected from the group consisting of avidin, strepavidin, an antibody that binds biotin and an antibody fragment that binds biotin.

43. The method of claim 42, wherein said host cell is an E. coli cell.

44. The method of claim 43, wherein said transformation step is accomplished by chemical transformation or electroporation.