Method of identifying hormone antagonists and agonists
First Claim
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1. A method of determining antagonist activity of a compound for a hormone receptor of the steroid hormone superfamily, comprising the steps of:
- combining said compound with said hormone receptor in vitro so as to induce a conformational change in said receptor, anddigesting said receptor with a proteolytic enzyme to detect said conformational change in said receptor, thereby measuring whether said compound is an antagonist of said receptor wherein the agonist-receptor fragment produced by the method is always greater than the antagonist receptor fragment.
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Abstract
The present invention provides a novel method of determining the antagonist and agonist activity of a compound for steroid hormone receptors. The present invention also provides a method of determining antagonist activity of a compound for a hormone receptor by inducing the conformational change in the receptor. In addition, the present invention provides a novel method of determining the level of agonist activity of a compound for steroid hormone receptors.
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6 Claims
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1. A method of determining antagonist activity of a compound for a hormone receptor of the steroid hormone superfamily, comprising the steps of:
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combining said compound with said hormone receptor in vitro so as to induce a conformational change in said receptor, and digesting said receptor with a proteolytic enzyme to detect said conformational change in said receptor, thereby measuring whether said compound is an antagonist of said receptor wherein the agonist-receptor fragment produced by the method is always greater than the antagonist receptor fragment. - View Dependent Claims (2, 3, 4, 5, 6)
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