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Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex

  • US 5,763,177 A
  • Filed: 03/08/1996
  • Issued: 06/09/1998
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. A method for identifying a nucleic acid ligand that photocrosslinks to a protein from a candidate mixture of nucleic acids, wherein each member of said candidate mixture contains a photoreactive group, said method comprising:

  • a) contacting said candidate mixture with said protein, wherein nucleic acids having an increased affinity to the protein relative to the candidate mixture form nucleic acid-protein complexes with the protein;

    b) irradiating said complexes, wherein said nucleic acid-protein photocrosslink;

    c) partitioning the photocrosslinked nucleic acid-protein complexes from in the candidate mixture; and

    d) identifying a nucleic acid ligand that photocrosslinked to the protein.

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