Fluorescence energy transfer and intramolecular energy transfer in particles using novel compounds
First Claim
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1. A composition made by a process comprising steps of:
- A) selecting at least one fluorescent hybrid phthalocyanine derivative containing a metal, said derivative(s) having (1) at least one donor subunit with a desired excitation peak; and
(2) at least one acceptor subunit with a desired emission peak; and
(3) at least one electron transfer subunit; and
(4) at least one axial ligand covalently bound to said metal in said phthalocyanine derivative;
wherein said derivative(s) is/are capable of intramolecular energy transfer from said donor subunit to said acceptor subunit; and
,B) randomly incorporating said phthalocyanine derivative(s) into a microparticle selected from the group consisting of latex, silica, alumina, liposomes and colloids.
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Abstract
Particles comprising an energy donor as a first component and a fluorescent dye as a second component positioned in said particles at an energy exchanging distance from one another, wherein the two components have a Stokes shift of greater than or equal to 50 nm, said particle having bound on its surface, a protein, polypeptide, nucleic acid, nucleotide or protein containing ligand analogue are disclosed and claimed. In addition, novel fluorescent dyes are described which exhibit intramolecular energy transfer for use to label various molecules, proteins, polypeptides, nucleotides and nucleic acids or to incorporate into particles.
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Citations
5 Claims
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1. A composition made by a process comprising steps of:
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A) selecting at least one fluorescent hybrid phthalocyanine derivative containing a metal, said derivative(s) having (1) at least one donor subunit with a desired excitation peak; and
(2) at least one acceptor subunit with a desired emission peak; and
(3) at least one electron transfer subunit; and
(4) at least one axial ligand covalently bound to said metal in said phthalocyanine derivative;
wherein said derivative(s) is/are capable of intramolecular energy transfer from said donor subunit to said acceptor subunit; and
,B) randomly incorporating said phthalocyanine derivative(s) into a microparticle selected from the group consisting of latex, silica, alumina, liposomes and colloids.
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2. A loadable microparticle comprising a fluorescent phthalocyanine derivative having a metal with at least one axial ligand covalently bound to said metal in said phthalocyanine derivative.
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3. A loadable microparticle comprising a fluorescent hybrid phthalocyanine derivative having a metal with at least one axial ligand covalently bound to said metal in said hybrid phthalocyanine derivative.
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4. A fluorescent microparticle made by the process comprising:
- A) selecting a series of fluorescent dyes comprising at least one initial donor dye with a desired excitation peak of greater than approximately 500 nm and two or more final acceptor dyes with desired emission peaks equal to or very similar to each other, wherein each dye in the series has a spectral overlap sufficient to allow for significant energy transfer of excitation energy to the final acceptor dyes and wherein a Stokes shift of treater than or equal to 50 nm exists between the energy donor and at least one of the energy acceptors, and at least one of said acceptor dyes has an emission wavelength greater than approximately 680 nm; and
B) randomly incorporating said series of dyes in a microparticle, whereby the improved particle exhibits minimal fluorescence quenching and maximum fluorescence intensity. - View Dependent Claims (5)
- A) selecting a series of fluorescent dyes comprising at least one initial donor dye with a desired excitation peak of greater than approximately 500 nm and two or more final acceptor dyes with desired emission peaks equal to or very similar to each other, wherein each dye in the series has a spectral overlap sufficient to allow for significant energy transfer of excitation energy to the final acceptor dyes and wherein a Stokes shift of treater than or equal to 50 nm exists between the energy donor and at least one of the energy acceptors, and at least one of said acceptor dyes has an emission wavelength greater than approximately 680 nm; and
Specification