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Inhibition of amylin release

  • US 5,763,200 A
  • Filed: 05/12/1995
  • Issued: 06/09/1998
  • Est. Priority Date: 05/12/1995
  • Status: Expired due to Fees
First Claim
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1. A method of determining the ability of a compound to both bind to somatostatin type-5 receptor and inhibit amylin release from amylin-secreting pancreas cells, said method comprising:

  • obtaining CHO-K1 cells transfected with DNA encoding the human somatostatin type-5 receptor or a membrane preparation obtained therefrom;

    incubating said CHO-K1 cells or a membrane preparation obtained therefrom, said compound, and a somatostatin type-5 receptor ligand, at least one of said ligand and said compound being detectably labeled;

    determining the ability of said compound to compete against said ligand for binding to somatostatin type-5 receptor;

    if and only if said compound binds to somatostatin type-5 receptor;

    obtaining amylin-secreting pancreatic cells;

    incubating said compound, said pancreatic cells, and an amylin release stimulator under conditions in which said amylin release stimulator would induce release of amylin from said pancreatic cells; and

    determining the ability of said compound to inhibit amylin release.

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