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Production and use of normalized DNA libraries

  • US 5,763,239 A
  • Filed: 06/18/1996
  • Issued: 06/09/1998
  • Est. Priority Date: 06/18/1996
  • Status: Expired due to Fees
First Claim
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1. A process for forming a normalized genomic DNA library from an environmental sample, which comprises the steps of:

  • (a) isolating a genomic DNA population from the environmental sample;

    (b) analyzing the complexity of the genomic DNA population so isolated;

    (c) at least one of the steps selected from the group consisting of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and

    (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.

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