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Enzyme capable of degrading cellulose or hemicellulose

  • US 5,763,254 A
  • Filed: 06/07/1995
  • Issued: 06/09/1998
  • Est. Priority Date: 05/09/1990
  • Status: Expired due to Term
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Assignment
First Claim
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1. An isolated and purified cellulose- or hemicellulose-degrading enzyme, comprising a catalytically active domain, a carbohydrate binding domain and a linking B region which operably links the catalytically active domain and the carbohydrate binding domain, wherein the carbohydrate binding domain has the following core sequence:

  • ##STR10## wherein;

    Xaa in position 1 is Trp or Tyr;

    Xaa in position 2 is Gly or Ala ;

    Xaa in position 7 is Gln, Ile or Asn;

    Xaa in position 8 is Gly or Asn;

    Xaa in position 9 is Trp, Phe or Tyr;

    Xaa in position 10 is Ser, Asn, Thr or Gln;

    Xaa in position 12 is Pro, Ala or Cys;

    Xaa in position 13 is Thr, Arg or Lys;

    Xaa in position 14 is Thr, Cys or Asn;

    Xaa in position 16 is one of the twenty standard L-α

    -amino acids found in proteins;

    Xaa in position 17 is one of the twenty standard L-α

    -amino acids found in proteins;

    Xaa in position 18 is Gly or Pro;

    Xaa in position 19 is Ser, Thr, Phe, Leu or Ala, or is absent;

    Xaa in position 20 is Thr or Lys;

    Xaa in position 22 is Val, Thr, Arg, Glu or Lys;

    Xaa in position 23 is Lys, Gln or Ala;

    Xaa in position 24 is Gln or Ile;

    Xaa in position 26 is Gln, Asp or Ala;

    Xaa in position 27 is Trp, Phe or Tyr;

    Xaa in position 29 is Tyr, Ser, His or Ala;

    Xaa in position 32 is Leu, Ile, Gln, Val or Thr; and

    Xaa in position 33 is one of the twenty standard L-α

    -amino acids found in proteins, or is absent;

    wherein the enzyme is endogenous to a strain of a fungus other than Trichoderma or Phanerochaete.

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