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Systematic evolution of ligands by exponential enrichment: Chemi-SELEX

  • US 5,763,595 A
  • Filed: 06/05/1995
  • Issued: 06/09/1998
  • Est. Priority Date: 06/11/1990
  • Status: Expired due to Term
First Claim
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1. Nucleic acid ligands which bind covalently with a protein, wherein said nucleic acid ligands comprise a chemically reactive functional unit selected from the group consisting of pohotoreactive groups and active site directed compounds, wherein said nucleic acid ligands are produced by a method comprising the steps of:

  • a) preparing a candidate mixture of nucleic acids;

    b) contacting said candidate mixture with said protein, wherein nucleic acids which bind covalently with said protein may be partitioned from the remainder of said candidate mixture;

    c) partitioning the nucleic acid-protein complexes from free nucleic acids in said candidate mixture; and

    d) identifying said nucleic acid ligands that bind covalently with said protein.

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