Apparatus for red blood cell separation

• US 5,766,552 A
• Filed: 02/05/1996
• Issued: 06/16/1998
• Est. Priority Date: 04/20/1993
• Status: Expired due to Fees

• Current Assignee: ActiMed Laboratories, Inc.
• Original Assignee: ActiMed Laboratories, Inc.
• Inventors: Law, Wai Tak, McGeehan, John K., Doshi, Sonal R.
• Primary Examiner(s): LUDLOW, JAN M
• Application Number:
  US08/596,828
• Time in Patent Office: 862 Days
• Field of Search: 435/7.25, 436/827, 436/526, 436/527, 436/531, 436/63, 436/177, 436/178, 422/61, 422/56-60, 422/101
• US Class Current: 422/535
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G01N 33/5002 Partitioning blood components
• CPC Class Codes: G01N 33/92 involving lipids, e.g. cholesterol , lipoproteins, or their receptors steroid hormones G01N33/743
• CPC Class Codes: Y10S 436/827 Lectins

Abstract

Red blood cells are removed from whole blood or a fraction thereof by agglutinating whole blood with a mixture of a free agglutinating agent and nucleating particles having agglutinating agent intimately associated therewith to form clusters of red blood cells. High molecular weight polyethylene glycol may be added further to enhance agglutination. The clusters of red blood cells are much larger than the size of individual red blood cells, so that the clusters can easily be filtered through a porous medium. The plasma, which is substantially free of red blood cells, is further passed through a filter that optionally contains an additional agglutinating agent. Flow-delay additives may be provided to retain the fluid sample in contact with a reagent for a predetermined time.

Claims

1. Apparatus for separation of red blood cells from whole blood comprising

a cluster forming means comprising the combination of a primary filter through which plasma or serum can pass, and a mixture of a free agglutinating agent and a particle-associated agglutinating agent intimately associated with nucleating particles.

2. Apparatus according to claim 1 wherein said free or particle-associated agglutinating agent comprises at least one lectin.

3. Apparatus according to claim 1 wherein at least 25% of said free or particle-associated agglutinating agent is particle-associated agglutinating agent and the remainder is free agglutinating agent.

4. Apparatus according to claim 1 wherein said primary filter is an absorbent pad and the cluster forming means is formed by incorporating said mixture into said pad.

5. Apparatus according to claim 1, wherein approximately 25% to 85% of said free or particle-associated agglutinating agent is free agglutinating agent and the remainder is particle-associated agglutinating agent.

6. Apparatus according to claim 1, wherein approximately 30% to 75% of said free or particle-associated agglutinating agent is free agglutinating agent and the remainder is particle-associated agglutinating agent.

7. Apparatus according to claim 1, wherein said cluster forming means further comprises polyethylene glycol having a molecular weight of about 10,000.

8. Apparatus according to claim 1 further comprising a secondary filter.

9. Apparatus according to claim 8 wherein said secondary filter contains a secondary agglutinating agent, wherein said secondary agglutinating agent is the same as or different from said free or particle-associated agglutinating agent.

10. Apparatus according to claim 8 wherein said secondary filter includes flow-delay means.

11. Apparatus according to claim 10 wherein said flow delay means comprises a coating of a flow-delay substance selected from the group consisting of alkyl ketene dimers, alkenyl succinic anhydrides, fluorocarbon resins, and mixtures thereof.

12. Apparatus according to claim 11 wherein said flow-delay substance further comprises at least one substance selected from the group consisting of saccharides and low molecular weight polymers.

13. Apparatus according to claim 11 wherein said nucleating particles are selected from the group consisting of particles made of polyacrolein, polyacrolein/iron oxide, latex, silica, glass, agarose, and mixtures thereof.

14. Apparatus according to claim 13 wherein said nucleating particles are particles of polyacrolein/iron oxide.

15. Apparatus according to claim 13 wherein said nucleating particles are particles of latex.

16. Apparatus according to claim 13 wherein said nucleating particles are glass particles.