Method for identification of high affinity nucleic acid ligands to selectins
First Claim
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1. A method for identifying nucleic acid ligands and nucleic acid ligand sequences to a selectin comprising:
- a) contacting a candidate mixture of nucleic acids with a selectin, wherein nucleic acids having an increased affinity to said selectin relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and
c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to said selectin, whereby nucleic acid ligands of said selectin may be identified.
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Abstract
This invention discloses high-affinity oligonucleotide ligands to lectins, specifically nucleic acid ligands having the ability to bind to the lectins, wheat germ agglutinin, L-selectin, E-selectin and P-selectin. Also disclosed are the methods for obtaining such ligands.
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Citations
9 Claims
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1. A method for identifying nucleic acid ligands and nucleic acid ligand sequences to a selectin comprising:
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a) contacting a candidate mixture of nucleic acids with a selectin, wherein nucleic acids having an increased affinity to said selectin relative to the candidate mixture may be partitioned from the remainder of the candidate mixture; b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture; and c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to said selectin, whereby nucleic acid ligands of said selectin may be identified. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification