Oligonucleotides containing base-free linking groups with photoactivatable side chains
First Claim
1. A nucleic acid compound comprising a nucleic acid sequence of at least 12 nucleotides defining a sequence of interest covalently linked at one end to a side chain characterized by having at least two nucleotides and not more than 8 nucleotides, having a photoactivatable group other than a nucleotide, wherein said photoactivatable group is bonded to a base-free linking group in said side chain.
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Abstract
Methods and compositions are provided for detecting nucleic acid sequences. In particular, pairs of probes are employed, where the pair defines a substantially contiguous sequence on a target nucleic acid. Each of the pairs has a side chain which forms a stem of the two side chains which non-covalently binds and is capable of forming a cross-link upon activation, when the probes and sample nucleic acid are base paired. Each of the nucleic acids is initially present as single stranded nucleic acid to allow for base pairing, so that the probes bind to homologous target nucleic acid. The assay mixture is activated to provide cross-linking, the double stranded nucleic acid melted, and the process of base pairing, activation and melting repeated, a sufficient number of cycles, to provide a detectable amount of cross-linked probes. Also provided are kits comprising reagents, as well as automatic devices, for carrying out the subject method.
50 Citations
7 Claims
- 1. A nucleic acid compound comprising a nucleic acid sequence of at least 12 nucleotides defining a sequence of interest covalently linked at one end to a side chain characterized by having at least two nucleotides and not more than 8 nucleotides, having a photoactivatable group other than a nucleotide, wherein said photoactivatable group is bonded to a base-free linking group in said side chain.
- 3. An oligonucleotide comprising from 2 to 20 nucleotides and a deoxyribosyl backbone having intervening in said backbone from 1 to 2 linkers comprising other than nucleosides and pendent from said linkers, a photoactivatable group other than a nucleotide, capable of forming a covalent bond with a nucleotide.
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5. An oligonucleotide compound comprising a first oligonucleotide unit comprising at least 14 nucleotides and a deoxyribosyl backbone having intervening in said backbone proximal to a first terminus of said first oligonucleotide unit from 1 to 2 linkers comprising other than nucleosides and pendent from said linkers, a photoactivatable group, other than a nucleotide, capable of forming a covalent bond with a nucleotide, said first terminus defining a side chain, and linked through said photoactivatable group a second oligonucleotide unit comprising at least 14 nucleotides and comprising a side chain having nucleotides complementary to and capable of base pairing with nucleotides in said first side chain at a second terminus opposite to said first terminus defining a complementary side chain, said first and second side chains forming a stem.
- 6. A kit comprising at least one pair of probes, said probes being characterized by having sequences homologous to adjacent portions of a target nucleic acid sequence and having side chains which non-covalently bind to form a stem upon base pairing of said probes to said target nucleic acid sequence, each of the members of said stem have at least two nucleotides which base pair to form said stem, at least one of said side chains having a photoactivatable group, which upon activation during stem formation forms a covalent cross-link with the other side chain member of said stem.
Specification