Endoscopic imaging system for diseased tissue

• US 5,769,792 A
• Filed: 04/15/1996
• Issued: 06/23/1998
• Est. Priority Date: 07/03/1991
• Status: Expired due to Term

• Current Assignee: Novadaq Technologies, Inc. (Stryker Corporation)
• Original Assignee: Xillix Technologies Corp.
• Inventors: Lam, Stephen C-T, Hung, Jaclyn Y-C, Profio, Amedeus E., MacAulay, Calum E., Palcic, Branko, Jaggi, Bruno W.
• Primary Examiner(s): Smith, Ruth S.
• Application Number:
  US08/632,018
• Time in Patent Office: 799 Days
• Field of Search: 128/633, 128/634, 128/664-666, 356/318, 250/341.1, 600/310, 600/317, 600/342, 600/473, 600/475-479
• US Class Current: 600/477
• CPC Class Codes: A61B 5/0071 by measuring fluorescence emission
• CPC Class Codes: A61B 5/0084 for introduction into the body, e.g. by catheters A61B5/1459 takes precedence
• CPC Class Codes: G01N 2021/6463 Optics

Abstract

Apparatus for imaging diseases in tissue comprising a light source for generating excitation light that includes wavelengths capable of generating characteristic autofluorescence for abnormal and normal tissue. A fiber-optic illuminating light guide is used to illuminate tissue with light that includes at least the excitation light thereby exciting the tissue to emit the characteristic autofluorescence. An imaging bundle collects emitted autofluorescence light from the tissue. The autofluorescence light is filtered into spectral bands in which the autofluorescence intensity for abnormal tissue is substantially different from normal tissue and the autofluorescence intensity for abnormal tissue is substantially similar to normal tissue. An optical system is used to intercept the filtered autofluorescence light to acquire at least two filtered emitted autofluorescence images of the tissue. The acquired images are displayed in real time on a display monitor in such a manner as to delineate abnormal and normal tissue.

Claims

1. Apparatus for imaging diseases in tissue using autofluorescence comprising:

a visible light source for generating excitation light that includes wavelengths that generate characteristic autofluorescence for abnormal and normal tissue;

means for illuminating tissue with light from the visible light source that includes at least said excitation light thereby exciting tissue to emit said characteristic autofluorescence;

collecting means for gathering reflected excitation light and emitted autofluorescence light from said tissue;

a dichroic mirror positioned to receive the reflected excitation light and the emitted autofluorescence light gathered by the collecting means, the dichroic mirror operating to separate the spectral components of said autofluorescence light into at least a first spectral band including the reflected excitation light and the emitted autofluorescence light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially different from normal tissue and a second spectral band different from said first spectral band including the emitted autofluorescernce light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially similar to normal tissue;

a first optical filter positioned to receive the light within the first spectral band, said first filter operating to remove the reflected excitation light from light within the first spectral band;

a second optical filter positioned to receive the light within the second spectral band;

a first detector array for receiving the autofluorescence light within the first spectral band and for producing a first autofluorescence image of the tissue;

a second detector array for receiving the autofluorescence light within the second spectral band and for producing a second autofluorescence image of the tissue; and

a color monitor that simultaneously displays the first and second autofluorescence images.

2. The apparatus of claim 1, in which the visible light source is a laser.

3. The apparatus of claim 1, in which the visible light source is a Xenon light source.

4. The apparatus of claim 1, wherein the dichroic mirror has a cutoff wavelength in the range of 600 nanometers.

5. The apparatus of claim 1, wherein the first optical filter is a band pass filter having a center frequency of about 500 nanometers and a band-pass of about ±

20 nanometers.

6. The apparatus of claim 1, wherein the second optical filter is a red, long pass filter having a cutoff frequency of 630 nanometers.

7. The apparatus of claim 1, in which the means for illuminating tissue is a fiber-optic light guide.

8. The apparatus of claim 7, in which the collecting means is an imaging bundle and a focusing lens disposed within the fiber-optic light guide.

9. Apparatus for imaging diseases in tissue using autofluorescence comprising:

a visible light source for generating excitation light that includes wavelengths that generate characteristic autofluorescence for abnormal and normal tissue;

means for illuminating tissue with light from the visible light source that includes at least said excitation light thereby exciting tissue to emit said characteristic autofluorescence;

collecting means for gathering a reflected excitation light and an emitted autofluorescence light from said tissue;

means for separating the spectral components of the collected reflected excitation light and the emitted autofluorescence light gathered by the collecting means, into at least a first spectral band including the reflected excitation light and the emitted autofluorescence light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially different from normal tissue and a second spectral band different from said first spectral band including the emitted autofluorescence light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially similar to normal tissue;

a first optical filter positioned to receive the light within the first spectral band, said first filter operating to remove the reflected excitation light from light within the first spectral band;

a second optical filter positioned to receive the light within the second spectral band;

a first CCD camera for receiving the autofluorescence light within the first spectral band and for producing a first autofluorescence image of the tissue;

a second CCD camera for receiving the autofluorescence light within the second spectral band and for producing a second autofluorescence image of the tissue; and

a color monitor that simultaneously displays the first and second autofluorescence image.

10. The apparatus of claim 9, wherein the means for separating the spectral components of the collected reflected excitation light and the autofluorescence light into the first and second spectral bands comprises a dichroic mirror.

11. The apparatus of claim 10, wherein the dichroic mirror has a cutoff wavelength of nearly 600 nanometers.

12. The apparatus of claim 9, wherein the means for separating the spectral components of the collected reflected excitation light and the autofluorescence light into the first and second spectral bands comprises a prism.

13. The apparatus of claim 9, in which the visible light source is a laser.

14. The apparatus of claim 9, in which the visible light source is a Xenon light source.

15. The apparatus of claim 9, wherein the first optical filter is a band pass filter having a center frequency of about 500 nanometers and a band-pass of about ±

20 nanometers.

16. The apparatus of claim 9, wherein the second optical filter is a red, long pass filter having a cutoff frequency of 630 nanometers.

17. The apparatus of claim 9, in which the means for illuminating tissue is a fiber-optic light guide.

18. The apparatus of claim 17, in which the collecting means is an imaging bundle and a focusing lens disposed within the fiber optic-light guide.

19. Apparatus for imaging diseases in tissue using autofluorescence comprising:

a visible light source for generating excitation light that includes wavelengths that generate characteristic autofluorescence for abnormal and normal tissue;

a fiber optic light guide for illuminating tissue with light from the visible light source that includes at least said excitation light thereby exciting tissue to emit said characteristic autofluorescence;

collecting means for gathering reflected excitation light and emitted autofluorescence light from said tissue;

a dichroic mirror positioned to receive the reflected excitation light and the emitted autofluorescence light gathered by the collecting means, the dichroic mirror operating to separate the spectral components of said autofluorescence light into at least a first spectral band including the reflected excitation light and the emitted autofluorescence light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially different from normal tissue and a second spectral band different from said first spectral band including the emitted autofluorescence light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially similar to normal tissue;

a first optical filter positioned to receive the light within the first spectral band, said first filter operating to remove the reflected excitation light from light within the first spectral band;

a first detector array for receiving the autofluorescence light within the first spectral band and for producing a first autofluorescence image of the tissue;

a second detector array for receiving the autofluorescence light within the second spectral band and for producing a second autofluorescence image of the tissue; and

a color monitor that simultaneously displays the first and second autofluorescence image.

20. The apparatus of claim 19, in which the visible light source is a laser.

21. The apparatus of claim 19, in which the visible light source is a Xenon light source.

22. The apparatus of claim 21, wherein the dichroic mirror has a cutoff wavelength in the range of 600 nanometers.

23. The apparatus of claim 22, wherein the first optical filter is a band pass filter having a center frequency of about 500 nanometers and a band-pass of about ±

20 nanometers.

24. The apparatus of claim 19, further comprising a second optical filter positioned to receive the light within the second spectral band.

25. The apparatus of claim 24, wherein the second filter is a red, long pass filter having a cutoff frequency of 630 nanometers.

26. The apparatus of claim 19, in which the collecting means is an imaging bundle and a focusing lens disposed within the fiber-optic light guide.

36. The apparatus of claim 25, in which the collecting means is an imaging bundle and a focusing lens disposed within the fiber optic-light guide.

27. Apparatus for imaging diseases in tissue using autofluorescence comprising:

a visible light source for generating excitation light that includes wavelengths that generaie characteristic autofluorescence for abnormal and normal tissue;

a fiber optic light guide for illuminating tissue with light from the visible light source that includes at least said excitation light thereby exciting tissue to emit said characteristic autofluorescence;

collecting means for gathering a reflected excitation light and an emitted autofluorescence light from said tissue;

means for separating the spectral components of the collected reflected excitation light and the emitted autofluorescence light gathered by the collecting means, into at least a first spectral band including the reflected excitation light and the emitted autofluorescence light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially different from normal tissue and a second spectral band different from said first spectral band including the emitted autofluorescence light having wavelengths where an autofluorescence intensity for abnormal tissue is substantially similar to normal tissue;

a first optical filter positioned to receive the light within the first spectral band, said first filter operating to remove the reflected excitation light from light within the first spectral band;

a first CCD camera for receiving the autofluorescence light within the first spectral band and for producing a first autofluorescence image of the tissue;

a second CCD camera for receiving the autofluorescence light within the second spectral band and for producing a second autofluorescence image of the tissue; and

a color monitor that simultaneously displays the first and second autofluorescence images.

28. The apparatus of claim 27, wherein the means for separating the spectral components of the collected reflected excitation light and the autofluorescence light into the first and second spectral bands comprises a dichroic mirror.

29. The apparatus of claim 28, wherein the dichroic mirror has a cutoff wavelength of nearly 600 nanometers.

30. The apparatus of claim 27, wherein the means for separating the spectral components of the collected reflected excitation light and the autofluorescence light into the first and secoirid spectral bands comprises a prism.

31. The apparatus of claim 27, in which the visible light source is a laser.

32. The apparatus of claim 27, in which the visible light source is a Xenon light source.

33. The apparatus of claim 32, wherein the first optical filter is a band pass filter having a center frequency of about 500 nanometers and a band-pass of about ±

20 nanometers.

34. The apparatus of claim 27, further comprising a second optical filter positioned to receive the light within the second spectral band.

35. The apparatus of claim 27, wherein the second optical filter is a red, long pass filter having a cutoff frequency of 630 nanometers.